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Apigenin alleviates renal cell injury and oxidative stress induced by high glucose by activating Keap1-Nrf2-ARE signaling pathway

Published on Jul. 02, 2024Total Views: 1167 times Total Downloads: 240 times Download Mobile

Author: CHEN Yijie 1 QUAN Zijiao 2 ZHENG Nan 3 XIE Yuequn 1

Affiliation: 1. Department of Emergency , Wenzhou People's Hospital, Wenzhou 325000, Zhejiang Province, China 2. College of Life and Environmental Sciences, Wenzhou University, Wenzhou 325000, Zhejiang Province, China 3. College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China

Keywords: Diabetic nephropathy Oxidative stress Cell injury Apigenin Keap1-Nrf2-ARE

DOI: 10.12173/j.issn.1008-049X.202310076

Reference: CHEN Yijie, QUAN Zijiao, ZHENG Nan, XIE Yuequn.Apigenin alleviates renal cell injury and oxidative stress induced by high glucose by activating Keap1-Nrf2-ARE signaling pathway[J].Zhongguo Yaoshi Zazhi,2024, 27(6):975-983.DOI: 10.12173/j.issn.1008-049X.202310076.[Article in Chinese]

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Abstract

Objective  To investigate the protective effect of apigenin on renal tubular epithelial cells (HK-2) induced by hyperglycemia and its potential mechanism.

Methods  HK-2 cells were incubated with d-glucose to establish an in vitro diabetic nephropathy model. Cell viability, apoptosis and oxidative stress were assessed. The mRNA levels of nuclear factor erythroid-2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1) and epoxy chloropropane Kelch sample related protein-1 (Keap1) were measured by quantitative real-time-PCR (q-PCR). Western blot analysis was performed to measure the protein expression of Nrf2.

Results  In HK-2 cells, high glucose decreased cell viability in a concentration- and time-dependent manner. Apigenin improved the viability of HK-2 cells under high glucose stress, reduced apoptosis and proinflammatory cytokine production, and inhibited the oxidative stress. Apigenin increased mRNA expression of Nrf2 and HO-1, increased expression of Nrf2 protein and reduced the mRNA level of Keap1. Inhibition of Nrf2 using small interfering RNA (siRNA) abolished the protective effects of apigenin on HK-2 cells under high glucose stress.

Conclusion  Apigenin treatment can alleviate the damage of HK-2 cells under high glucose stress, and exert the anti-oxidation and anti-inflammation effects through Keap1-Nrf2-ARE pathway.

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