Objective  To improve the drug properties of insoluble drug resveratrol through nanotechnology, carry out the prescription design of resveratrol nanoemulsion (RES-NE), preliminarily evaluate the quality evaluation, and study its effect on preventing skin damage caused by ultraviolet rays.

Methods  The solubility of resveratrol in different excipients was investigated, and the proportion of auxiliary materials was determined by drawing ternary phase diagrams to prepare resveratrol nanoemulsions. The physicochemical properties such as particle size, Zeta potential, and morphology of resveratrol nanoemulsion were characterized. The protective effect of resveratrol nanoemulsion on the back skin of rats was investigated by ultraviolet irradiation.

Results   According to the solubility and ternary phase diagram, it was determined that glyceryl triacetate was the oil phase, polyoxyethylene castor oil EL-60 was emulsifier, polyethylene glycol 400 was used as co-emulsifier, the optimal formulation of resveratrol nanoemulsion was the drug, oil phase, emulsifier and co-emulsifier, with the mass ratio of 0.175  ∶  1.4 ∶ 2.0 ∶ 0.6, and the volume of ultrapure water was 25 mL. The particle size of the prepared nanoemulsion was (21.68±3.89) nm, the polydispersity index was 0.110±0.010, and the Zeta potential was (0.509±0.145) mV. Large areas of erythema appeared in the ultraviolet radiation control group, and no or very small areas of erythema appeared in the medication group.

Conclusion  The prepared resveratrol nanoemulsion preparation has clear appearance, safe and stable nature, simple preparation process, high drug load, and certain slow-release effect. It is preliminarily believed that resveratrol has the potential to prevent skin damage caused by ultraviolet rays, and is expected to be used in skin care, ultraviolet protection and other fields.

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