Objective  To establish the HPLC fingerprint of Physochlainae radix, and to predict the efficacy related substances of Physochlainae radix by chemometrics and network pharmacology.

Methods  The fingerprint was established by HPLC method, and the common peaks were matched and identified. The "composition-target-pathway" network was constructed, and the active ingredients were screened according to the connectivity. The samples were classified by chemometrics methods to predict potential efficacy related substances.

Results  A total of 14 common peaks were calibrated, among which 5 were confirmed by comparison with reference substances. It was preliminarily predicted that hyoscyamine, anisodamine, scopolin and scopoletin may achieve  the effect of relieving cough and asthma by regulating multiple targets such as PIK3CA, AKT2 as well as human cytomegalovirus infection, VEGF signaling pathway, relaxin signaling pathway.

Conclusion  In this study, it was found that the 4 components were closely related to the efficacy properties of Physochlainae radix, which can provide a reference for further research on the mechanism of action of Physochlainae radix.

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