Objective  To establish ultra performance liquid chromatography (UPLC) specific chromatogram of Gleditsiae spina, determine the contents of scopoletin and taxifolin, and provide reference for comprehensively evaluating the quality of Gleditsiae spina.

Methods  The UPLC method was used to establish the specific chromatogram of Gleditsiae spina and determine the contents of two active components, scopoletin and taxifolin. According to the specific chromatogram and component determination results, clustering analysis was performed on 17 batches of Gleditsiae spina, and comparative analysis was performed on medicinal materials from different origins and common counterfeits.

Results  The specific chromatogram of Gleditsiae spina was established, with 8 characteristic peaks identified. After mass spectrometry analysis and comparison with reference substances, it was confirmed that the components of 6 characteristic peaks were protocatechualdehyde, scopolin, scopoletin, taxifolin, vitexin and isovitexin. The 17 batches of Gleditsiae spina were divided into two categories, the content of Gleditsiae spina was higher in Luoyang, Henan province, and the content of taxifolin in Gleditsiae spina was lower in Shaanxi Province and Sichuan Province. The method of characteristic spectrum and content determination can be used to quickly and effectively distinguish the common 4 counterfeits.

Conclusion  This method can provides reference for establishing a comprehensive quality standard for Gleditsiae spina and comprehensively evaluating its quality.

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