Objective  To establish a method for comprehensive quality evaluation of Tongjing ointment by multi-component quantification combined with chemometrics and grey correlation analysis (GRA).

Methods  With curcumin as the internal reference substance, HPLC-QAMS method was used to simultaneously determine the contents of limonin, evodiamine, rutaecarpine, bisdemethoxycurcumin, demethoxycurcumin, curcumin, 6-gingerol, 8-gingerol, 10-gingerol, tetrahydropalmatine, dehydrocorydalin, dorydaline in Tongjing ointment. The quality of Tongjing ointment was evaluated by cluster analysis, principal component analysis, orthogonal partial least square discriminant analysis and GRA.

Results The determination of 12 components manifested a good linear relationship in the range of mass concentration (r≥0.999 1). The average recovery was between 96.58% and 100.19% (RSD<2.0%, n=9). There were no significant difference between the measured value of external standard method and the calculated value of HPLC-QAMS (P>0.05). Tongjing ointment samples were classified into three eategories by chemometrics and it showed that curcumin, 6-gingerol, bisdemethoxycurcumin, demethoxycurcumin, limonin and tetrahydropalmatine were the main potential markers affecting the quality of Tongjing ointment. GRA showed that the relative correlation degree was in the range of 0.317 3-0.624 8, and there were some differences in the quality of Tongjing ointment.

Conclusion  The established method can comprehensively evaluate the quality of Tongjing ointment.

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