Objective  To optimize extraction technology for Sihuang Shaoshang ointment by orthogonal test, and establish the HPLC fingerprint of Sihuang Shaoshang ointment to provide references for the quality evaluation of the preparation.

Methods  Taking Sihuang Shaoshang ointment as the model drug, the fingerprints were established by HPLC method, the common peak areas were analyzed by principal component analysis, and the total factor scores were used as the evaluation index. The technological parameters such as extraction times, extraction time and liquid-solid ratio were optimized by orthogonal test, and the optimal extraction process parameters were screened out.

Results  The common mode of fingerprint was set up with 12 common peaks and the two principle components with the accumulative contribution rate of 84.751% extracted by principal component analysis were screened out to calculate the comprehensive scores of 9 samples. The optimal extraction process was as follows: 10-fold amount of water, extracting 3 times, and extracting 2 h for each time.

Conclusion  The established method is simple and effective, which can provide a reference for the extraction process optimization of Sihuang Shaoshang ointment.

1.刘梦, 姚丽伟, 陈淑云, 等. 二苯乙烯苷通过APOE- TREM2介导的抗炎机制研究[J]. 中药新药与临床药理, 2020, 31(7): 755-761. [Liu M, Yao LW, Chen SY, et al. Study on the anti-inflammatory mechanism of TSG mechanism of TSG mediated by APOE-TREM2[J]. Traditional Chinese Drug Research and Clinical Pharmacology, 2020, 31(7): 755-761.] DOI: 10.19378/j.issn.1003-9783.2020.07.002.

2.陈子卓, 徐宇航, 赵九洲, 等. 二苯乙烯苷抗氧化和抗炎作用的机制研究[J]. 癌变·畸变·突变, 2020, 32(2): 105-111, 117. [Chen ZZ, Xu YH, Zhao JZ, et al. Antioxidant and anti-inflammatory effects of 2, 3, 45, 4'-tetrahydroxystilbene-2-O-β-D-glucoside in macrophages[J]. Carcinogenesis,Teratogenesis & Mutagenesis, 2020, 32(2): 105-111, 117.] DOI: 10.3969/j.issn.1004-616x.2020.02.005.

3.吴婉琴. 合欢皮抗焦虑活性部位作用机理研究及主要活性成分对PC12细胞凋亡的影响[D]. 武汉: 湖北中医药大学, 2017.

4.于鲁志. 中药甘草抗炎作用药理和临床研究进展[J]. 光明中医, 2017, 32(19): 2895-2898. [Yu LZ. Pharmacodynamics and clinical research progress on anti-inflammatory effect of licorice[J]. Guangming Journal of Chinese Medicine, 2017, 32(19): 2895-2898.] DOI: 10.3969/j.issn.1003-8914.2017.19.065.

5.邓桃妹, 彭灿, 彭代银, 等. 甘草化学成分和药理作用研究进展及质量标志物的探讨[J]. 中国中药杂志, 2021, 46(11): 2660-2676. [Deng TM, Peng C, Peng DY, et al. Research progress on chemical constituents and pharmacological effects of Glycyrrhizae Radix et Rhizoma and discussion of Q-markers[J]. China Journal of Chinese Materia Medica, 2021, 46(11): 2660-2676.] DOI: 10.19540/j.cnki.cjcmm.20210304.201.

6.黄娇, 罗杰伟, 韩丽, 等. 甘草酸铵调控IL-33/ST2通路对特应性皮炎小鼠肥大细胞活化的影响[J]. 药物评价研究, 2020, 43(11): 2181-2186. [Huang J, Luo JW, Han L, et al. Effect of ammonium glycyrrhizinate regulation of IL-33/ST2 pathway on mast cell activation in a topic dermatitis mice[J]. Drug Evaluation Research, 2020, 43(11): 2181-2186.] DOI: 10.7501/j.issn.1674-6376.2020.11.006.

7.中国药典2020年版. 一部[J]. 2020: 88, 130, 150, 277.

8.裴科, 宁燕, 蔡皓, 等. 基于HPLC指纹图谱结合化学模式识别的川芎炮制前后对比研究[J]. 中草药, 2021, 52(5): 1274-1283. [Pei K, Ning Y, Cai H, et al. Comparative study of Chuanxiong Rhizoma before and after processing based on HPLC fingerprints combined with chemical pattern recognition[J]. Chinese Traditional and Herbal Drugs, 2021, 52(5): 1274-1283.] DOI: 10.7501/j.issn.0253-2670.2021.05.007.

9.张志鹏, 梁丽金, 徐杰, 等. 巴戟天及根皮与木心UPLC特征指纹图谱与化学模式识别方法研究[J]. 中草药, 2020, 51(13): 3554-3560. [Zhang ZP, Liang LJ, Xu J, et al. Identification of morindae officinalis radix root cortex and woody core based on UPLC characteristic chromatogram and chemical pattern recognition method[J]. Chinese Traditional and Herbal Drugs, 2020, 51(13): 3554-3560.] DOI: 10.7501/j.issn.0253-2670.2020. 13.025.

10.干丽, 严玉晶, 丁青, 等. 基于指纹图谱和多成分含量测定的白芍与炒白芍标准汤剂质量差异性研究[J]. 中国中药杂志, 2021, 46(6): 1410-1416. [Gan L, Yan YJ, Ding Q, et al. Research on quality difference of standard decoction of raw and fried Paeoniae Radix Alba based on fingerprint and multicomponent determination[J]. China Journal of Chinese Materia Medica, 2021, 46(6): 1410-1416.] DOI: 10.19540/j.cnki.cjcmm.20201130.301.

11.周丽, 时海燕, 时银萍, 等. 指纹图谱结合化学计量学优选经典名方温胆汤的提取工艺[J]. 中国医院药学杂志, 2020, 40(21): 2214-2219. [Zhou L, Shi HY, Shi YP, et al. Optimization of extraction technology of classical Mingfang Wendan decotion by fingerprint combined with chemometrics[J]. Chinese Journal of Hospital Pharmacy, 2020, 40(21): 2214-2219.] DOI: 10.13286/j.1001-5213. 2020.21.02.

12.王璐璐, 刘炳周, 王满, 等. 主成分分析结合均匀设计法用于优选桂芍巴布贴制备工艺的研究[J]. 中草药, 2016, 47(10): 1682-1689. [Wang LL, Liu BZ, Wang M, et al. Optimization of preparation process of Gui-Shao cataplasm based on principal component analysis combined with uniform design[J]. Chinese Traditional and Herbal Drugs, 2016, 47(10): 1682-1689.] DOI: 10.7501/j.issn.0253-2670.2016.10.010.

13.邓明慧, 路娟, 柴瑞平, 等. 基于指纹图谱、聚类分析、主成分分析评价不同产地桑叶质量[J]. 时珍国医国药, 2020, 31(5): 1139-1142. [Deng MH, Lu J, Cai RP, et al. Quality evaluation of mori folium from different origins based on fingerprint, cluster analysis and principal component analysis[J]. Lishizhen Medicine and Materia Medica Research, 2020, 31(5): 1139-1142.] DOI: 10.3969/j.issn.1008-0805.2020.05.031.

14.杜晓娟, 张振秋, 杨燕云, 等. 多指标正交设计优选白芍甘草(炙)药对提取物的最佳提取工艺[J]. 辽宁中医药大学学报, 2016, 18(2): 32-34. [Du XJ, Zhang ZQ, Yang YY, et al. Study on extracting technology for paeoniae radix alba matched glycyrrhizae radix et rhizoma cum melle by orthogonal test [J]. Journal of Liaoning University of Traditional Chinese Medicine2016, 18(2): 32-34.] DOI: 10.13194/j.issn.1673-842x.2016.02.010.

15.贾成友, 李微, 张传辉, 等. 基于多指标权重分析和正交设计法优选白黄泄热止痢片复方提取工艺[J]. 中草药, 2016, 47(6): 917-922. [Jia CY, Li W, Zhang CH, et al. Exploration on extraction process of compound Baihuang Xiere Zhili tablets based on orthogonal design and multi-index weight analysis[J]. Chinese Traditional and Herbal Drugs, 2016, 47(6): 917-922.] DOI: 10.7501/j.issn.0253- 2670.2016.06.010.

16.李钦青, 柴金苗, 贺文彬, 等. 高效液相色谱法同时测定复方藿枝颗粒中淫羊藿苷、宝藿苷Ⅰ、甘草苷和甘草酸铵的含量[J]. 中药新药与临床药理, 2020, 31(6): 714-717. [Li QQ, Cai JM, He WB, et al. Simultaneous determination of icariin, baohuoside Ⅰ, liquiritin and ammonium glycyrrhizinatein in Fufang Huozhi granule by HPLC[J]. Traditional Chinese Drug Research and Clinical Pharmacology, 2020, 31(6): 714-717.] DOI: 10.19378/j.issn.1003-9783.2020.06.014.

17.候新莲, 黄露, 彭成, 等. HPLC指纹图谱结合正交试验优化红参的炮制工艺[J]. 中国药房, 2020, 31(10): 1228-1232. [Hou XL, Huang L, Peng C, et al. Optimization of processing technology of Ginseng Radix et Rhizoma Rubra by HPLC fingerprint combined with orthogonal test[J]. China Pharmacy, 2020, 31(10): 1228-1232.] DOI: 10.6039/j.issn.1001-0408.2020.10.14.

18.Liu MQ, Wu YJ, Huang SS, et al. Spectrum-effect relationship between HPLC fingerprints and hypoglycemic effect of Curcuma aromatica[J]. Biomed Chromatogr, 2018, 32(7): 4220. DOI: 10.1002/bmc.4220.

19.林秀敏, 张振凌, 王胜超, 等. 白芍饮片的HPLC指纹图谱建立及聚类分析、主成分分析[J]. 中国药房, 2019, 30(24): 3375-3382. [Lin XM, Zhang ZL, Wang SC, et al. Establishment of HPLC fingerprints of Paeonia tactilora decoction pieces and its cluster analysis and principal component analysis[J]. China Pharmacy, 2019, 30(24): 3375-3382.] DOI: CNKI:SUN:ZGYA.0.2019-24-015.