Objective  To optimize the extraction process of damp-removing weight loss paste.

Methods  The active ingredients of damp-removing weight loss paste were predicted using network pharmacology, and the binding ability between active ingredients and core targets was verified by molecular docking technology. Based on the compatibility rules and pharmacological effects of various herbs in the prescription, the process evaluation indicators of damp-removing weight loss paste were determined. The weight coefficients of each indicator were determined by the combination of analytic hierarchy process and the entropy weight method, and the comprehensive score was used as the final evaluation indicator. The extraction process was optimized through single-factor experiments and Box-Behnken response surface methodology.

Results The main active components of damp-removing weight loss paste were naringin, hesperidin, neohesperidin, nuciferine, and naringenin, and the molecular docking results indicated that these active ingredients had strong binding affinities with the core target (<-5.0 kcal/mol). The weight coefficients of naringin, hesperidin, neohesperidin, nuciferine, and naringenin were 0.191 8, 0.281  2, 0.113  0, 0.323 8, and 0.090 2, respectively. The optimal extraction process was as follows: decocting with 10-fold water for 2 times, each lasting 1.5 h. The verification experiments showed that the quantified contents of the 5 target components were in good agreement with the predicted values. The RSD calculated from 3 parallel experiments were all below 5%.

Conclusion The extraction process of damp-removing weight loss paste is stable and feasible, and can lay the foundation for further in-depth research.

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