HPLC fingerprint combined with chemical pattern recognition to evaluate the differences between decoction and formula granules of Niaodukang

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Author: Qing-Hong HU ¹ Xiao-Jie LI ¹ Yuan XU ² Jie DING ³ Ming-Lan ZHAO ¹ Hua LIN ⁴  Jun-Biao WU ⁴

Affiliation: 1. The Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, China 2. Department of Nephrology, the Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510120, China 3. Department of Research, the Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510120, China 4. Department of Pharmacy, the Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510120, China

Keywords: Niaodukang Decoction Formula granule HPLC Characteristic chromatograms Similarity evaluation Chemical pattern recognition

DOI: 10.12173/j.issn.1008-049X.202302173

Reference: Qing-Hong HU, Xiao-Jie LI, Yuan XU, Jie DING, Ming-Lan ZHAO, Hua LIN,Jun-Biao WU.HPLC fingerprint combined with chemical pattern recognition to evaluate the differences between decoction and formula granules of Niaodukang[J].Zhongguo Yaoshi Zazhi,2023, 26(10):12-21.DOI: 10.12173/j.issn.1008-049X.202302173.[Article in Chinese]  Copied

Abstract
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Abstract

Objective To establish an HPLC fingerprint of Niaodukang, compare the differences between Chinese herbal decoction and formula granules (FG) with chemical pattern recognition, and provide a theoretical basis for the equivalence study.

Methods The analysis was performed on Thermo 5005-254630 C₁₈ (250 mm × 4.6 mm, 5 μm) column with acetonitrile-0.1% phosphoric acid solution for gradient elution at the flow rate of 0.8 mL·min^-1, the detection wavelength was 254 nm, the column temperature was 22℃, and the injection volume was 20 μL. The HPLC fingerprints of 9 batches of Niaodukang were established, then the similarity of chromatograms was investigated by the Chinese medicine chromatographic fingerprint similarity evaluation system, combined hierarchical cluster analysis (HCA), principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), and the variable importance projection value (VIP) >1 was used as a standard to comprehensively evaluate the difference among the traditional decoction(TD), formula granule mixed (FGm), and formula granule decocted (FGd) of Niaodukang.

Results HPLC fingerprints of 9 batches of Niaodukang were established with similarity between 0.754-0.993. 21 common peaks were calibrated, and 8 chromatographic peaks were identified. The samples were classified into two clusters by HCA, PCA and OPLS-DA, and seven markers that caused the difference among batches were screened, which were peak 3, peak 1, peak 5 (calycosin-7-O-β-D-glucopyranoside), peak 7 (nvzhenoside), peak 16 (aloe emodin), peak 13 (cinnamaldehyde) and peak 2.

Conclusion There are differences in chemical composition between Niaodukang decoction and formula granules. FGm and FGd had good similarity, Meanwhile, screening out the difference components could provide a basis for process optimization and quality evaluation.

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