Objective  To investigate the effect of Huangzhi oral liquid on ferroptosis and apoptosis of hepatocytes in acute hyperlipidemia mice based on abnormal lipid metabolism.

Methods  Forty SPF grade C57BL/6 mice were randomly assigned to the normal control group, the model group, Huangzhi oral liquid low-dose and high-dose groups, and fenofibrate group, with 8 mice in each group. All administration groups were given Huangzhi oral liquid and fenofibrate by gavage at corresponding doses, while the normal control group and the model group were given the same dose of distilled water for a period of 5 days. on the third day of administration, in addition to the normal control group, other groups were injected intramuscularly with Triton WR-1339 to establish a hyperlipidemia model. After the  administration, the serum lipid levels and liver function in mice were detected. The pathological changes of liver were observed by HE staining and lipid accumulation was observed by oil red O staining. The iron content in liver tissues was detected by tissue iron assay kit. The changes in protein expression of ABCA1, ABCG5, LXRα, ASGR1, cleaved-PARP, cleaved-caspase 3, Bcl2, Bax, TFR1, SLC7A11, GCLM, GPX4 and FTH1 in liver were detected by Western Blot.

Results  Compared with the model group, Huangzhi oral liquid could reduce the lipid vacuoles in liver tissues, alleviate the degree of lipid degeneration, reduce the content of TC, TG, LDL-C, AST and ALT in serum, increase the content of HDL-C, increase the protein expression levels of ABCA1, ABCG5, LXRα, Bcl-2, SLC7A11, GCLM, GPX4 and FTH1 in mice liver tissues, reduce the protein expression levels of ASGR1, cleaved-PARP, cleaved-caspase 3, Bax and TFR1.

Conclusion  Huangzhi oral liquid can alleviate the abnormal lipid metabolism in acute hyperlipidemia mice, thus alleviates hepatocyte apoptosis and ferroptosis.

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