Objective  To analyze the molecular mechanism of Yupingfeng granules (YPFG) in the treatment of recurrent respiratory tract infection (RRTI), and provide a pharmacological basis for the diagnosis and treatment of RRTI in traditional Chinese medicine.

Methods  The TCMSP database and SwissTargetPredication database were used to screen the active ingredients and active ingredient targets of YPFG. All pathogenic targets of RRTI were obtained using the DisGeNET dataBase, DrugBank database, GeneCards database, and OMIM database. Drug-disease target-compound networks and protein-protein interaction networks were established to explore the main drugs, compounds, and target proteins. GO enrichment analysis and KEGG signaling pathway enrichment analysis were used to explore the main molecular mechanisms of YPFG in the treatment of RRTI.

Results  The main active ingredients of YPFG screened include three main active ingredients, including daylily lignans A, peucedanum praeruptorum B, and wogonin, which mainly target SRC and COX-2. Enrichment analysis showed that phosphorylation (biological process), cytoplasm perinuclear region (cellular component), and phosphotransferase activity (molecular function) were the main GO enrichment items. The signaling pathways mainly involve tumor signaling pathways, relaxin signaling pathways, Th17 signaling pathways, and so on.

Conclusion  YPFG mainly treats RRTI by participating in the signal transduction of certain key nodes in tumor signaling pathways, relaxin signaling pathways, Th17 signaling pathways, etc. It has a direct or indirect impact on multiple signaling pathways and has the characteristics of multi-component, multi-target, and multi-channel effects.

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