Objective  To explore the optimal extraction process of flavonoids from Epimedii folium by deep eutectic solvent method.

Methods  Using the extraction rate of epimedin A, epimedin B, epimedin C and icariin in Epimedii folium as evaluation indices, the extraction process of flavonoids in Epimedii folium was optimized based on single factor experiments such as solid-liquid ratio, molar ratio, moisture content, extraction temperature, and extraction time, combined with orthogonal experimental design, and the components in the solvent were recovered using macroporous resin.

Results  The deep eutectic solvent formed by choline chloride and ethanolamine had the best extraction effect. When the molar ratio of choline chloride to ethanolamine was 1 ∶ 4, the solid-liquid ratio was 0.5 g ∶ 25 mL, the water content was 50%, the extraction temperature was 70 ℃, and the extraction time was 15 minutes, the total extraction rate was (20.07±0.43) mg/g, and the AB-8 macroporous resin could recover flavonoids in the eutectic solvent with an average recovery rate of 88.29%.

Conclusion  Deep eutectic solvents have great potential in the extraction of active ingredients in traditional Chinese medicine due to their simple preparation and higher extraction efficiency compared to traditional solvents.

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