Quality evaluation of Clematidis radix et rhizoma medicinal materials based on HPLC-QAMS multi-components quantification combined with OPLS-DA and weighted TOPSIS method

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Author: FENG Xiaochuan ^1, ^2, ³ ZHANG Jing ^1, ^2, ³ XU Yanzhao ⁴  ZHANG Rui ^1, ^2, ³

Affiliation: 1. Department of Chinese Pharmacy, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China 2. The Fourth Clinical Medical College of Peking university, Beijing 100035, China 3. National Center for Orthopaedics, Beijing 100035, China 4. National Institutes for Food and Drug Control, Beijing 100050, China

Keywords: Clematidis radix et rhizoma High-performance liquid chromatography Stechiometry Principal component analysis Orthogonal partial least squares discriminant analysis Weighted TOPSIS method Quality evaluation

DOI: 10.12173/j.issn.2097-4922.202406118

Reference: FANG Xiaoyang, ZHOU Rongrong, SHEN Bingbing, HE Weiwei, XIAO Jie, XU Jin, ZENG Hongliang, ZHANG Shuihan.Quality evaluation of Clematidis radix et rhizoma medicinal materials based on HPLC-QAMS multi-components quantification combined with OPLS-DA and weighted TOPSIS method[J].Yaoxue QianYan Zazhi,2024, 28(4):593-604.DOI: 10.12173/j.issn.2097-4922.202406118.[Article in Chinese]  Copied

Abstract
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Abstract

Objective To establish a method for simultaneous detection of 9 components in Clematidis radix et rhizoma from different producing areas, screen the differential markers affecting their quality, and to evaluate its quality difference.

Methods Reflux extraction was performed on 16 batches of Clematidis radix et rhizoma from 8 provinces, and the extracts were detected by HPLC. The chemical identification model and weighted TOPSIS method were used to establish the quality evaluation model of Clematidis radix et rhizoma, and the quality difference was comprehensively evaluated.

Results Clematichinenoside AR, huzhangoside D, clematichinenoside C, huzhangoside B, hederagenin, oleanolic acid, 3,5,6,7,8,3',4'-heptamethoxyflavone, hesperetin and formononetin showed good linear relationships within the ranges of 1.27-31.75, 4.48-112.00, 7.35-183.75, 3.69-92.25, 6.16-154.00, 20.95-523.75, 0.58-14.50, 0.39-9.75 and 0.26-6.50 µg/mL (r>0.999 0), respectively. The average recovery rate was 96.91%-100.12%, and the RSD was 0.71%-1.53% (n=9). 16 batches of samples were grouped into 3 categories, and oleanolic acid, clematichinenoside C, clematichinenoside AR and hederagenin might be the main potential markers affecting the quality of Clematidis radix et rhizoma. The analysis results of the weighted TOPSIS method revealed that the closeness for evaluating the quality of 16 batches of Clematidis radix et rhizoma ranged from 0.097 8 to 0.818 2, with S13 achieving the highest value of 0.818 2.

Conclusion The method for quantitative analysis of 9 components in Clematidis radix et rhizoma is simple and accurate. Chemometrics and weighted TOPSIS method can be used to evaluate the quality difference.

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