Comparison of extraction methods of main components of Rubiae radix et rhizoma and study of the changes before and after processing

Published on Jan. 05, 2025Total Views: 467 times Total Downloads: 67 times Download Mobile

Author: LU Yuanxun ¹  ZHANG Zhewen ¹ CHEN Hongjiang ²  LUO Yiyuan ²

Affiliation: 1. Ningbo Ming Bei China Pharmaceutical Co., LTD, Ningbo 315192, Zhejiang Province, China 2. College of Traditional Chinese Medicine, Zhejiang Pharmaceutical University, Ningbo 315500, Zhejiang Province, China

Keywords: Rubiae radix et rhizoma Content determination Purpurin Rubimaillin Decoction piece processing Inspection standards

DOI: 10.12173/j.issn.2097-4922.202405050

Reference: LU Yuanxun, ZHANG Zhewen, CHEN Hongjiang, LUO Yiyuan.Comparison of extraction methods of main components of Rubiae radix et rhizoma and study of the changes before and after processing[J].Yaoxue QianYan Zazhi,2024, 28(4):617-622.DOI: 10.12173/j.issn.2097-4922.202405050. [Article in Chinese]  Copied

Abstract
Full-text
References

Abstract

Objective To compare the impacts of different extraction techniques on the primary components of Rubiae radix et rhizoma and investigate the variations in the major chemical constituents before and after processing.

Methods The concentrations of purpurin and rubimaillin were assayed using the methods prescribed in the 2020 edition of the Chinese Pharmacopoeia (pharmacopoeia method) and an optimized method (improved method). The changes in these constituents were then compared and analyzed.

Results The acid extraction method was adopted to simplify the alcohol extraction and acid hydrolysis process recommended by the pharmacopoeia method, leading to a more efficient and less time-consuming procedure. The results of methodological investigation showed that the stability of the solution was good within 24 h. The average recovery rates of purpurin and rubimaillin were in the range of 102.12%-104.95% (with the RSD of 1.13%) and 93.78%-95.49% (with the RSD of 0.80%), respectively (n=6). The determination results of pharmacopoeia method showed that the content of purpurin decreased, while the content of rubiarin increased after processing, which was not completely consistent with the actual trends. The improved method significantly improved the extraction efficiency of purpurin, thus providing a more accurate reflection of the total purpurin content in Rubiae radix et rhizoma.

Conclusion The established method is accurate, simple and effective, which provides reference for the reasonable formulation of inspection standards and quality control of madder.

Full-text

Please download the PDF version to read the full text: download

References

1. 中国药典2020年版. 一部[S]. 2020: 245-246.

2. 陈毅, 王海丽, 薛露, 等. 茜草的研究进展[J]. 中草药, 2017, 48(13): 2771-2779. [Chen Y, Wang HL, Xue L, et al. Research progress on Rubiae Radix et Rhizoma[J]. Chinese Traditional and Herbal Drugs, 2017, 48(13): 2771-2779.] DOI: 10.7501/j.issn.0253-2670.2017.13.029.

3. 张琳, 胡本祥, 安衍茹, 等. 茜草本草考证[J]. 吉林中医药, 2018, 38(3): 317-320. [Zhang L, Hu BX, An YR, et al. Herbalogical study on Rubiae Radix et Rhizoma[J]. Jilin Journal of Traditional Chinese Medicine, 2018, 38(3): 317-320.] DOI: 10.13463/j.cnki.jlzyy.2018.03.020.

4. 中国药典2000年版. 一部[S]. 2000: 187-188.

5. 中国药典2005年版. 一部[S]. 2005: 162.

6. 中国药典2010年版. 一部[S]. 2010: 218-219.

7. 中国药典2015年版. 一部[S]. 2015: 234-235.

8. 姜云, 乔春凤, 刘华, 等. HPLC多指标成分定量联合化学计量学评价不同产地茜草[J]. 现代药物与临床, 2024, 39(1): 94-101. [Jiang Y, Qiao CF, Liu H, et al. Evaluation of Rubiae Radix from different regions by HPLC multi indexcomponents combined with chemometrics[J].Drugs & Clinic, 2024, 39(1): 94-101.] DOI: 10.7501/j.issn.1674-5515.2024.01.012.

9. 李欣, 高天鹏, 张明童, 等. 基于多组分含量测定及多元统计分析研究茜草、大茜草及大叶茜草[J]. 药物分析杂志, 2023, 43(12): 2072-2080. [Li X, Gao TP, Zhang MT, et al. Based on multi-component content determination andmultivariate statistical analysis study Rubiae Radix et Rhizoma, Rubia magna P.G. Hsiao. and Rubia schumanniana Pritz[J]. Chinese Journal of Pharmaceutical Analysis, 2023, 43(12): 2072-2080.] DOI: 10.16155/j.0254-1793.2023.12.12.

10. 郭桂明, 蔡乐, 梁小雨, 等. HPLC法测定茜草饮片中大叶茜草素和羟基茜草素的含量[J]. 北京中医药, 2011, 30(7): 541-543. [Guo GM, Cai L, Liang XY, et al. Determination of rubitin and hydroxyl rubitin in Rubiae Radix et Rhizoma decoction slices by HPLC[J]. Beijing Journal of Traditional Chinese Medicine, 2011, 30(7): 541-543.] DOI: 10.16025/j.1674-1307.2011.07.027.

11. 王锐, 曹赢, 王知斌. “一测多评”法测定茜草中3种有效成分百分含量[J]. 哈尔滨商业大学学报(自然科学版), 2016, 32(4): 426-428, 432. [Wang R, Cao Y, Wang ZB. Determination of three active ingredients in Radix Rubiae by quantitative analysis of multi-components with single-marker[J]. Journal of Harbin University of Commerce (Natural Sciences Edition), 2016, 32(4): 426-428, 432.] DOI: 10.19492/j.cnki.1672-0946.2016.04.011.

12. 于瑞, 高明洁, 崔彬彬, 等. HPLC法测定中药茜草中茜草素、羟基茜草素和大叶茜草素的含量[J]. 哈尔滨医科大学学报, 2017, 51(3): 195-199. [Yu R, Gao MJ, Cui BB, et al. Simultaneous determination of alizarin, purpurin and mollugin in Rubia cordifolia L. by HPLC[J]. Journal of Harbin Medical University, 2017, 51(3): 195-199.] DOI: 10.3969/j.issn.1000-1905.2017.03.002.

13. 杨丽. 对2015年版《中国药典》中茜草定量测定方法的改进[J]. 中成药, 2020, 42(9): 2527-2529. DOI: 10. 3969/j.issn.1001-1528.2020.09.054.

14. 林文华, 朱喜梅, 严萍, 等. 茜草中大叶茜草素、羟基茜草素含量测定方法优化的实验研究[J]. 中药新药与临床药理, 2014, 25(3): 345-348. [Lin WH, Zhu XM, Yan P, et al. Experiment research on optimization of determination method for rubimaillin and purpurin in Radix Rubiae[J]. Traditional Chinese Drug Research and Clinical Pharmacology, 2014, 25(3): 345-348.] DOI: 10.3969/j.issn.1003-9783.2014.03.027.

15. 杨宇婷, 康文艺. HPLC法测定茜草及不同炮制品中大叶茜草素[J]. 中成药, 2011, 33(12): 2125-2127. [Yang YT, Kang WY. Determination of rubimaillin contents in Rubia Cordifolia L. and different processed products by HPLC[J]. Chinese Traditional Patent Medicine, 2011, 33(12): 2125-2127.] DOI: 10.3969/j.issn.1001-1528.2011.12.028.