Objective To conduct the qualitative analysis of the chemical composition and mobile components in rat blood of Jiegu Qili tablets using ultra performance liquid chromatography-quadrupole-exactive-mass spectrometry (UPLC-Q-Exactive-MS) technology, and to provide reference for the in vivo study of effective substances in this preparation.

Methods In vitro chromatographic condition was as follows: Hypersil GOLD C₁₈ chromatography column (50 mm × 2.1 mm, 1.9 µm) was used, the mobile phase was 0.1% formic acid aqueous solution and methanol (gradient elution), the column temperature was 30  ℃, and the injection volume was 5  µL. In vitro mass spectrometry condition was as follows: the sample was ionized by electric spray and detected by positive and negative ion dual mode, with scanning range of m/z 100~1 500. In vivo chromatographic condition was as follows: ACQUITY HPLC HSS T3 chromatography column (100 mm×2.1 mm, 1.8 µm) was used, the mobile phase was 5% water+5% acetonitrile and 47.5% acetonitrile+47.5% isopropanol+5% water (gradient elution), the column temperature was 40 ℃, and the injection volume was 8  µL. In vivo mass spectrometry condition was as follows: the sample was ionized by electric spray and detected by positive and negative ion dual mode, with scanning range of m/z 70~1 050. SD rats were given Jiegu Qili tablets by intragastric administration for 7 days, and drug-containing serum and blank serum samples (female and male Jiegu Qili tablets serum group, and female and male blank serum group) were obtained through abdominal aorta. The non-targeted LC-MS technique and Progenesis QI software were used for comparative analysis to identify the transitional components in the blood of rats.

Results  75 compounds were identified in vitro, and 98 compounds were identified in vivo. Based on the chemical composition identified in vitro and in vivo, a total of 7 blood components were identified, including methyl isoquercetin, arachidonic acid, citric acid, pyroglutamic acid, 3-indole propionic acid, 16 hydroxypalmitic acid, and oleic acid amide.

Conclusion  This method is efficient, accurate and sensitive, and can provide scientific reference for future research on the pharmacological substance basis and quality control of Jiegu Qili tablets

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