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Home Articles Vol 30,2026 No.4 Detail

Comparative study of the pharmacokinetics of Abelmoschus manihot (L.) Medic. flowers in directly taken decoction pieces and traditional decoction pieces of hyperoside in normal and nephrotic syndrome rats

Published on May. 01, 2026Total Views: 48 times Total Downloads: 11 times Download Mobile

Author: NIU Kailin 1 LUO Heng 2 ZHOU Jing 2, 3 BAI Xiaolu 2 WEN Qing 2, 3 CHEN Chu 2 WU Yan 2 ZHANG Hong 1 MENG Huix-in 1 ZHANG Lei 2, 3

Affiliation: 1. College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China 2. Lanboratory Animal Center, Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China 3. Sichuan-Chongqing Joint Key Laboratory of Innovation of New Drugs of Traditional Chinese Medicine, Chengdu 610041, China

Keywords: Abelmoschus manihot (L.) Medic. Hyperoside Nephrotic syndrome Directly taken decoction pieces Traditional decoction pieces HPLC-QqQ/MS Pharmacokinetics Drug metabolism Rats

DOI: 10.12173/j.issn.2097-4922.202504070

Reference: NIU Kailin, LUO Heng, ZHOU Jing, BAI Xiaolu, WEN Qing, CHEN Chu, WU Yan, ZHANG Hong, MENG Huixin, ZHANG Lei. Comparative study of the pharmacokinetics of Abelmoschus manihot (L.) Medic. flowers in directly taken decoction pieces and traditional decoction pieces of hyperoside in normal and nephrotic syndrome rats[J]. Yaoxue QianYan Zazhi, 2026, 30(4): 595-602. DOI: 10.12173/j.issn.2097-4922.202504070.[Article in Chinese]

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Abstract

Objective To establish high-performance liquid chromatography-tandem quadrupole mass spectrometry (HPLC-QqQ/MS) for the determination of the content of hyperoside in rat plasma, and to compare and analyze the pharmacokinetic characteristics of hyperoside in Abelmoschus manihot (L.) Medic. flowers directly taken decoction pieces and traditional decoction pieces in normal and nephrotic syndrome (NS) rats.

Methods After the plasma was extracted with ethyl acetate, the separation was performed on an Agilent Poroshell 120 EC C18 chromatographic column (100 mm×3 mm, 2.7 µm) , and the mobile phase was methanol 0.1% formic acid aqueous solution with a gradient elution. The detection was conducted in the negative ion mode of electric spray ion source. The pharmacokinetic parameters such as peak time (Tmax), peak concentration (Cmax), area under the drug time curve (AUC), mean drug retention time (MRT), clearance rate (CL/F) and other parameters were calculated using the DAS 2.0 software.

Results  Compared with the traditional decoction pieces, oral administration of the direct decoction pieces significantly shortened Tmax and increased Cmax in normal rats, whereas Cmax was significantly decreased in NS model rats (P<0.05). Compared with normal rats, NS model rats showed significantly increased AUC₀–t and AUC₀–∞, along with decreased V/F and CL/F following oral administration of both direct and traditional decoction pieces (P<0.05). In addition, Tmax was markedly prolonged and MRT₀–t was significantly extended after administration of the direct decoction pieces in NS model rats (P<0.05). After administration of the traditional decoction pieces, Cmax was significantly increased in NS model rats (P<0.01).

Conclusion  The pharmacokinetic profiles of hyperoside differed markedly between normal and NS model rats following oral administration of both direct and traditional decoction pieces of Abelmoschus manihot. In normal rats, the direct decoction pieces exhibited faster absorption and a shorter Tmax. In contrast, in NS model rats, the direct decoction pieces resulted in a lower peak plasma concentration and reduced fluctuation, suggesting a better safety profile compared with traditional decoction pieces. Moreover, the NS model significantly increased AUC₀–t and AUC₀–∞, while decreasing CL/F and V/F, which may be associated with impaired renal clearance and altered plasma protein binding.

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