Objective  To determine 10 components in Shiliang tea by high performance liquid chromatography-quantitative analysis of multi-components with a single-marker (HPLC-QAMS), and to evaluate its comprehensive quality by multivariate statistical analysis and entropy-technique for order preference by similarity to ideal solution (E-TOPSIS).

Methods  The determination was performed on COSMOSIL®5 C18-MS-Ⅱ column (250 mm×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution and gradient elution at the flow rate of 1.0 mL·min^-1, and the detection wavelength was set at 360 nm and 222 nm. The contents of scopolin, scopoletin, isofraxidin, rutoside, kaempferol-3-O-rutinoside, astragalin, scoparone, quercetin, kaempferol and mcalycanthine in 18 batches of Shiliang tea were calculated according to QAMS using rutoside as internal reference. The content data of 18 batches of Shiliang tea were analyzed chemometrically using statistical software; the quality of Shiliang tea was evaluated comprehensively using E-TOPSIS method.

Results  Good separation was obtained for all 10 components and showed good linearity with peak area in their respective scopes (r>0.999 0). The average recovery rates were 96.98%-100.12% (RSD<2.0%, n=9). The average relative correction factors for rutoside and the other 9 components were 2.115 7, 2.592 4, 0.553 1, 0.897 6, 0.780 7, 1.159 3, 0.693 6, 1.458 3 and 0.601 7 (RSD<2.0%, n=6), and there was no significant difference between the contents obtained by QAMS and external standard method for each component. The results of multivariate statistical analysis showed that the cumulative variance contribution rate of the 2 principal components was 83.886%, and the variable importance in the projection value greater than 1 were kaempferol-3-O-rutinoside, rutoside and astragalin. The results of E-TOPSIS showed that the euclidean closeness of the optimal solution was between 0.180 4 and 0.739 4.

Conclusion  The method established is simple, accurate, economical and practical, which can fully reflect the quality difference of Shiliang tea and better control the quality of this variety.

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