Objective To establish and compare the UPLC characteristic chromatograms of Sinapis alba L., Brassica juncea (L.) Czern. et Coss and their processed products.

Methods The characteristic chromatograms of Sinapis alba L., Brassica juncea (L.) Czern. et Coss and their processed products were determined by UPLC method, and chemometric pattern recognition analysis was performed based on the characteristic chromatograms.

Results There were significant differences in the characteristic chromatograms. Peak 4 (sinapine thiocyanate), peak 5, and peak 8 (sinapic acid) were common characteristic peaks of Sinapis alba L., Brassica juncea (L.) Czern. et Coss and their processed products. Peak 1 (sinalbin), peak 2 (p-hydroxybenzyl alcohol), peak 3, peak 6 and peak 7 were specific components of Sinapis alba L., while peak 11 (4-hydroxyglucobrassicin) and peak 12-16 were specific components of Brassica juncea (L.) Czern. et Coss. In addition, Peak 2, Peak 9 and Peak 10 were important markers for distinguishing Sinapis alba and its processed products. Peak 11 served as a key marker for distinguishing Brassica juncea and its processed products.

Conclusion The UPLC characteristic chromatogram method established in this study enables the discrimination of Sinapis alba L., Brassica juncea (L.) Czern. et Coss and their processed products, providing a basis for the quality control of Sinapis alba L., Brassica juncea (L.) Czern. et Coss and their processed products.

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