Objective  To establish HPLC fingerprints of soaking Euodiae fructus with water decoction of Radix glycyrrhizae by Zhangbang method (hereinafter referred to as “soaking Euodiae fructus”), and to determine the content of evodiamine, rutaecarpine and evocarpine, so as to provide the basis for the quality control and standard improvement of soaking Euodiae fructus.

Methods  The fingerprint of soaking Euodiae fructus was established based on wavelength switching technology, and the similarity evaluation was conducted. Taking the rutaecarpine as the internal reference, the relative correction factors of evodiamine and evocarpine were calculated by slope correction method, and the differences in measurement results between quantitative analysis of multi-components by single-marker (QAMS) method and external standard method were compared.

Results  The established fingerprint of soaking Euodiae fructus had a total of 20 common peaks, the similarity between it and the control fingerprint spectrum was 0.970 to 0.998, and 7 common peaks of them were identified. The contents of evodiamine and evocarpine determined by QAMS method were 1.754-7.542 mg/ g and 1.281-2.455 mg/g in 10 batches of samples, and the results obtained by QAMS method and external standard method were similar.

Conclusion  The established HPLC method is simple, reliable, with good separation and repeatability, and can be used for quality evaluation of processed Euodiae fructus.

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