Objective  To analyze the dynamic changes and correlation of color characteristics and fingerprints of HPLC during processing of Sophora alopecuroides L.

Methods  HPLC was used to establish the fingerprint spectrum of Sophora alopecuroides L. during processing and the changes of its main components were analyzed. The color eigenvalus in the processing of Sophora alopecuroides L. were extracted, and cluster analysis was conducted; correlation analysis between the characteristic values of the samples and the fingerprint chromatographic peaks were carried out.

Results  With the prolongation of the processing time, the similarity of the Sophora alopecuroides L. fingerprint gradually decreased, common peaks such as N-methylphenanthine, sophoridine, matrine, sophorine, etc. increased first and then decreased. The red (R), green(G) and intensity(I) color eigenvalues decreased with the extension of processing time, while the hue (H) eigenvalue gradually increased with the extension of processing time. The correlation analysis of color eigenvalus and fingerprint peaks showed that the common peaks 1 and peaks 5-11 of fingerprints were significantly correlated with color characteristics. The results of partial least squares discriminant analysis showed that sophoridine, matrine, sophorine, etc. were the main indexes of quality change in the processing of Sophora alopecuroides L.

Conclusion  The chemical composition of Sophora alopecuroides L. is constantly changing during processing, and the color eigenvalus of Sophora alopecuroides  L. at different processing times are closely related to HPLC fingerprints, which can provide reference for attenuating the toxicity, maximizing the clinical efficacy and evaluating the quality of Sophora alopecuroides L...

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