Study on the chemical constituents and in vitro anti-tumor cell activity of Phlomoides ambigua

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Author: CHEN Dan YANG Shimei YANG Surong ZHANG Yingchen QI Yanyan  LI Ganpeng

Affiliation: Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan Minzu University, Kunming 650500, China

Keywords: Phlomoides ambigua Chemical constituents Iridoids Phenylethanoid glycoside Anti-tumor cell activity

DOI: 10.12173/j.issn.2097-4922.202412102

Reference: CHEN Dan, YANG Shimei, YANG Surong, ZHANG Yingchen, QI Yanyan, LI Ganpeng. Study on the chemical constituents and in vitro anti-tumor cell activity of Phlomoides ambigua[J]. Yaoxue QianYan Zazhi, 2025, 29(6): 924-933. DOI: 10.12173/j.issn.2097-4922.202412102.[Article in Chinese]  Copied

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Abstract

Objective To study on the chemical constituents and in vitro anti-tumor cell activity of Phlomoides ambigua.

Methods The chemical constituents of Phlomoides ambigua 95% ethanol aqueous extract and 90% petroleum ether-10% chloroform mixture extract were separated by positive and reverse phase silica gel columns, Sephadex LH-20 gel columns, HPLC and other chromatography techniques. The structures of the separated compounds were characterized by nuclear magnetic resonance, mass spectrometry, infrared spectroscopy, ultraviolet spectroscopy etc, and the CCK-8 assay was utilized to assess the anti-tumor cell activity of compounds.

Results A total 18 compounds were isolated and identified from the extract of Phlomoides ambigua including salvialoside A, acteoside, luteolin-7-O-β-D-glucopyranoside, alyssonoside, barlerin, 5,9-epi-7,8-didehydropenstemoside(6), sesamoside(7), myristic acid(8), daucosterol(9), compound VI(10), ethyl-β-D-glucopyranoside(11), 6,9-epi-8-O-acetyl shanziside methyl ester(12), shanzhiside methyl ester(13), 5,9-epi-phlomiol(14), phlomiol(15), 5,9-epi-penstemoside(16), lariciresinol-9-O-β-D-glucopyranoside(17), brazilin(18). Compounds 14, 15 and 16 exhibited inhibitory effects on HCT-15 at various concentrations (40, 8, 1.6, 0.32, 0.064 μmol/L). The inhibition rate increased with the increasing drug concentration. Among them, compound 14 showed an half maximal inhibitory concentration of (5.86±0.77) μmol/L.

Conclusion Compounds 10, 17 and 18 are isolated for the first time in this genus. Compounds 1~18 are isolated from Phlomoides ambigua for the first time. Compound 14 can inhibit the proliferation of HCT-15, demonstrating a certain antitumor activity.

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