Objective  To establish a quality evaluation method for Zhike Huatan mixture by integrating fingerprint with chemometrics.

Methods HPLC method was used, the chromatographic column was Shim pack GIST C₁₈ column (250 mm×4.6 mm, 5 μm), the mobile phase was 0.1% formic acid aqueous solution acetonitrile (gradient elution), the flow rate was 1.0 mL/min, the detection wavelength was 327 nm, the column temperature was 30℃, and the injection volume was 10 μL. The fingerprints of Zhike Huatan mixture were established, the common peaks were confirmed through comparative analysis, and the chemical composition identification was performed on the main chromatographic peaks. The fingerprints were systematically analyzed using chemometric methods.

Results  Fingerprints for 20 batches of Zhike Huatan mixture were established and 34 common peaks were confirmed, and 6 chromatographic peaks were identified by comparison with reference substances, which were forsythoside A, liquiritin, hesperidin, baicalin, nobiletin, and praeruptorin A. The fingerprint similarity between 20 batches of Zhike Huatan mixture had reached 0.99 or above. Through systematic chemometric analysis (including cluster analysis, principal component analysis and partial least squares discriminant analysis), it was found that liquiritin, forsythoside A, baicalin and praeruptorin A were the key indicator components affecting the quality of Zhike Huatan mixture.

Conclusion  The established method by HPLC fingerprint combined with chemometric analysis is stable and reliable, providing scientific basis for the quality control and comprehensive evaluation of Zhike Huatan mixture.

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