Fufangansiliheji is a commonly used ethnic medicine hospital preparation in the Hetian region, known for its anti-inflammatory, cough-suppressing, expectorant, and anti-asthmatic effects. It is clinically applied for respiratory diseases such as bronchial asthma. Currently, this preparation lacks a systematic and comprehensive quality control system, particularly missing quality evaluation indicators that reflect its characteristic “multi-component synergistic effects.” Based on its formula composition and incorporating the core concept of quality markers(Q-Markers), this paper explores the screening and identification of its Q-Markers from multiple dimensions, including quality transfer and traceability, component specificity, component effectiveness, formula compatibility, component measurability, and network pharmacology. Preliminary screening has identified various components as potential Q-Markers for Fufangansiliheji, including flavonoids (genkwanin, apigenin, luteolin, diosmetin, tilianin), phenolic acids (rosmarinic acid, chlorogenic acid), and terpenoids (eburicoic acid, 3-keto-dehydrotrametenolic acid, linalool, carvone, α-asarone). This aims to provide a reference for establishing and enhancing its quality standards.

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