Optimization of extraction process for Chuanhu Qutong granules

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Author: WANG Yonggang ^1, ² HAO Haoqi ^1, ² LIU Zhenyuan ^1, ² WANG Pengfei ^1, ² ZHENG Xuejie ^1, ² ZHANG Cuiyun ^1, ² HE Fengcheng ^1, ²  FAN Jianwei ^1, ²  GUAN Yongxia ^1, ²

Affiliation: 1.Lunan Hope Pharmaceutical Co., Ltd., Linyi 276006, Shandong Province, China 2.State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Lunan Pharmaceutical Group Co., Ltd., Linyi 276006, Shandong Province, China

Keywords: Chuanhu Qutong granules Orthogonal experimental design Emodin Transfer rate Extract yield Extraction process

DOI: 10.12173/j.issn.2097-4922.202605100

Abstract
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Abstract

Objective To optimize the extraction process of Chuanhu Qutong granules.

Methods Key factors including liquid-to-material ratio, soaking time, decoction time were evaluated. Emodin transfer rate and extract yield were used as comprehensive evaluation indices. In addition, specific TLC identification of Dioscorea nipponica was performed to track the dissolution state of diosgenin saponin components. An L₉ (3⁴) orthogonal experimental design was applied to optimize the extraction process, followed by validation.

Results The optimal conditions were determined as 6-fold volume of water, 40 minutes of soaking, two decoctions, and 1 hour per decoction. Under these conditions, the average emodin transfer rate in three batches of samples prepared under this process was 18.12%, with an average extract yield of 20.21%. The TLC spots of Dioscorea nipponica were clear with good reproducibility.

Conclusion The optimized process is stable and feasible, and can be applied to the preparation of Chuanhu Qutong granules.

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