Inhibition of lupinol on the malignant progression of lung cancer A549 cells through regulating Notch signaling pathway

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Author: YIN Bingyi ¹  LIN Hongsheng ¹ ZHANG Chuchu ²

Affiliation: 1. Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China 2. Institute of Traditional Chinese Medicine Information, China Academy of Chinese Medical Sciences, Beijing 100091, China

Keywords: Lupeol Notch signaling pathway Lung cancer cell Proliferation Apoptosis Migration Invasion

DOI: 10.12173/j.issn.1008-049X.202403208

Reference: YIN Bingyi, LIN Hongsheng, ZHANG Chuchu.Inhibition of lupinol on the malignant progression of lung cancer A549 cells through regulating Notch signaling pathway[J].Zhongguo Yaoshi Zazhi,2024, 27(6):961-968.DOI: 10.12173/j.issn.1008-049X.202403208.[Article in Chinese]  Copied

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Abstract

Objective To explore the mechanism of lupinol on the proliferation, apoptosis, migration and invasion of lung cancer cells through regulating Notch signaling pathway.

Methods Lung cancer cells A549 were cultured in vitro, and the cells were treated with lupeol at concentrations of 0, 15, 30, and 60 mg/L, with 0 mg/L being the control group and the rese being the lupeol dosage groups, the cells were treated with lupinol at the concentration of 60 mg/L and Notch pathway inhibitor DAPT at the concentration of 10 μmol/ L, which was recorded as the lupeol+DAPT group. Cell proliferation changes were detected by MTT; cell apoptosis was detected by flow cytometry; cell migration and invasion were detected by Transwell; protein expression of PCNA, Bcl-2, N-cadherin, E-cadherin, Notch-1 and Hes- 1 were detected by Western blot.

Results 15, 30, 60 mg/L lupeol group can significantly inhibit the cell viability, the number of migration cells and invading cells of lung cancer cells, significantly increase the rate of cell apoptosis, and reduce PCNA, N-cadherin, Bcl-2, Hes-1 and Notch-1 expression, increase E-cadherin expression (P<0.05). Compared with the lupeol group, the lupeol+DAPT group significantly reduced cell viability, the number of migrating cells and invaded cells, increased apoptosis rate, decreased PCNA, Bcl-2, Hes-1, Notch-1 and N-cadherin protein expression, and increased E-cadherin protein expression (P<0.05).

Conclusion Lupinol may inhibit the invasion, migration and proliferation of lung cancer cells through Notch signaling pathway, and induce apoptosis.

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