Study on optimization of the extraction process of Perillae fructus formula granules by Box-Behnken response surface methodology

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Author: LU Lihe ^1, ² HUANG Leguan ^1, ² DONG Wenjie ^1, ² YANG Yan ^1, ² NIU Yanfei ³ YANG Zengming ^1, ² HU Diqiang ^1, ² FENG Liping ^1, ²  TANG Panpan ^1, ²

Affiliation: 1. Yunnan Institute of Materia Medica, Kunming 650111, China 2. Yunnan Provincial Key Laboratory for TCM and Ethnic Drug of New Drug Creation, Kunming 650111, China 3. Yunnan Baiyao Group Chinese Medicinal Resources Division, Kunming 650501, China

Keywords: Perillae fructus Formula granules Box-Behnken response surface method Extraction process

DOI: 10.12173/j.issn.2097-4922.202409102

Reference: LU Lihe, HUANG Leguan, DONG Wenjie, YANG Yan, NIU Yanfei, YANG Zengming, HU Diqiang, FENG Liping, TANG Panpan. Study on optimization of the extraction process of Perillae fructus formula granules by Box-Behnken response surface methodology[J]. Yaoxue QianYan Zazhi, 2025, 29(3): 398-405. DOI: 10.12173/j.issn.2097-4922.202409102.[Article in Chinese]  Copied

Abstract
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Abstract

Objective To optimize the extraction process of Perillae fructus formula granules.

Methods On the basis of single factor experiments, the extraction process of Perillae fructus formula granules was optimized using Box Behnken response surface methodology, with extraction time, solid-liquid ratio and extraction times as investigation factors, rosmarinic acid content and paste yield as evaluation indicators. The dry granulation of extract powder was explored.

Results The optimized preparation process of Perillae fructus formula granules was as follows: boiling with 12 times the amount of water for 90 min, concentrating and dring under reduced pressure to produce paste powder, adding an appropriate amount of maltodextrin, silica, etc., mixing well, dring to form granules, and producing qualified finished Perillae fructus formula granules.

Conclusion The optimized preparation process is simple, stable and feasible, providing a basis for industrial production of Perillae fructus formula granules.

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