Objective  To establish a method for determining the content of inulin-type fructan in Codonopsis pilosula, and provide a reference for comprehensive quality evaluation of Codonopsis pilosula.

Methods  The reference substance of inulin-type fructan from Codonopsis pilosula was prepared using ultrafiltration membrane technology, and a quantitative analysis method based on high-performance gel permeation chromatography (HPGPC) was established. The content of inulin-type fructan in 20 batches of Codonopsis pilosula from different origins was determined, and a comparative analysis of the quality of medicinal materials from different origins was conducted.

Results  In this study, a method for the determination of inulin-type fructan content in Codonopsis pilosula was established with inulin-type fructan from Codonopsis pilosula as the control substance. The established method met the requirements of methodological validation, showing a good linear relationship within the range of 2.00-10.00 µg ( r≥0.999 9) . The average recovery rate was 101.12% (RSD=2.65%, n=9). Significant differences were observed in the content of inulin-type fructan among Codonopsis pilosula from different origins. The average content of inulin-type fructan from Shanxi (8.43%±3.10%) was significantly higher than that in Gansu samples (3.52%±0.36%).

Conclusion  This method can be used for the determination of inulin-type fructan in Codonopsis pilosula, and demonstrates good repeatability and accuracy, which provides scientific evidence for improving the quality evaluation system of Codonopsis pilosula..

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