Objective  To establish ultra-performance liquid chromatography (UPLC) fingerprint method and multi-index constituents content determination method for Serissa serissoides, and provide a basis for its quality evaluation.

Methods  The fingerprint of Serissa serissoides was established by UPLC technology, and the quality differences of different batches of Serissa serissoides were comprehensively evaluated through similarity evaluation and multivariate statistical analysis, combined with the determination of the content of five indicator components. The UPLC chromatographic condition was as follows: HSS T3 column (150 mm×2.1 mm, 1.8 μm) was used, the mobile phase was acetonitrile -0.1% phosphoric acid (gradient elution), the flow rate was 0.3  mL/min, the column temperature was 30 ℃, the detection wavelength was 236 nm, and the injection volume was 1 μL.

Results  The linear relationship of each component was good (r≥0.999 5), with an average recovery rate of 97.05%~102.48% (RSD<2.0%, n=6). The fingerprints of 13 batches of Serissa serissoides samples were established, a total of 15 common peaks were calibrated, and 5 of these chemical components were identified. The similarity results of 13 batches of samples showed that, except for 1 batch with a similarity of 0.795, all other batches were not less than 0.851. The results of hierarchical cluster analysis and principal component analysis were basically consistent, dividing the 13 batches of medicinal materials into 3 categories. By combining the principle of variable importance in the projection>1, 7 main differential characteristic components responsible for the inter-batch differences were screened out.

Conclusion  The UPLC fingerprint and multi-index constituents determination method established in this study is rapid, simple, accurate, reliable, and reproducible, which can provide a basis for the quality control and evaluation of Serissa serissoides. The research results indicate that there are significant differences in the composition of Serissa serissoides medicinal materials from different origins and batches. The results show that there are significant differences in the component contents of Serissa serissoides among different origins and batches.

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