Objective  To establish a HPLC method for the simultaneous determination of hederagenin, oleanolic acid, ursolic acid, scabioside C, protocatechuic acid, chlorogenic acid, caffeic acid, aesculetin, scopoletin, rutoside, quercetin, kaempferol in Patrina scabiosaefolia Fisch, and to explore the application of principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA) and grey relational analysis (GRA) in the quality evaluation of Patrina scabiosaefolia Fisch.

Methods  The HPLC method was used with the Waters Atlantis T3 C₁₈ column (250 mm×4.6 mm, 5 µm). The mobile phase was acetonitrile- 0.1% phosphate acid solution with gradient elution. The detection wavelengths were 210, 260 and 360 nm. The overall quality of Patrina scabiosaefolia Fisch was comprehensively evaluated by PCA, OPLS-DA and GRA analysis of the results of multi-component content.

Results  The results of methodological verification of external standard method met the requirements. The 12 components had good linear relationships within their respective ranges (r>0.999 0), and the average recovery rate were 96.82%-100.16% (RSD<2.0%, n=9). PCA and OPLS-DA results showed that oleanolic acid, ursolic acid, chlorogenic acid and quercetin were the main potential markers affecting the quality of Patrina scabiosaefolia Fisch. The relative correlation degree of GRA were 0.380 6-0.571 4, and there was a certain difference between batches of Patrina scabiosaefolia Fisch.

Conclusion  The HPLC method can simultaneously determine the 12 components in Patrina scabiosaefolia Fisch, which is simple and practical, and PCA, OPLS-DA and GRA can be used to evaluate the quality of Patrina scabiosaefolia Fisch.

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