Objective To establish a method for determining the residues of paclobutrazol and ochratoxin A in Yangyin Qingfei syrup, and to evaluate potential safety risk factors of this preparation.

Methods High-performance liquid chromatography with fluorescence detection (HPLC-FLD) combined with an immunoaffinity column was used for the quantitative screening of ochratoxin A, while liquid chromatography-mass spectrometry (LC-MS) was employed to determine the residue of paclobutrazol. A safety assessment of Yangyin Qingfei syrup was subsequently conducted.

Results Among the 54 samples, ochratoxin A was detected in 24 batches, but all concentrations were below the proposed limit. The residue of paclobutrazol exceeded the proposed limit in 26 batches, which was analyzed to be introduced by the raw material ofOphiopogon japonicus.

Conclusion The established method accurately detected the residues of paclobutrazol and ochratoxin A in Yangyin Qingfei syrup. Relevant manufacturers should pay attention to the safety risks associated with mycotoxin contamination and pesticide residues.

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