Objective To explore the changes in volatile components among four processed products of raw Cuscutae Semen, stir-fried Cuscutae Semen, salt-processed CuscutaeSemen, and wine-processed Cuscutae Semen ,and to screen the differential components among different processed products.

Methods The odor and volatile components of different processed products of Cuscutae Semen were identified using an electronic nose and GC-MS. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were conducted using Origin2024 software and SIMCA 14.1 software. According to the principle of variable importance in projection (VIP) value > 1 and P < 0.05, the differential components before and after the processing of Cuscutae Semen were screened.

Results The electronic nose detected that the four processed products of Cuscutae Semen were sensitive to alcohols, aldehydes, and ketones. A total of 89 volatile components were detected by GC-MS, including 49 in raw Cuscuta chinensis, 66 in stir-fried Cuscutae Semen, 56 in salt-processed Cuscutae Semen, and 65 in wine-processed Cuscutae Semen. Compared with raw Cuscuta chinensis, stir-fried Cuscuta chinensis had 27 newly added compounds and 10 reduced compounds. Salt-processed Cuscuta chinensis had 17 newly added compounds and 10 reduced compounds, while wine-processed Cuscuta chinensis had 32 newly added compounds and 16 reduced compounds. OPLS-DA revealed significant differences in volatile components among the different processed products of Cuscutae Semen, and a total of 12 differential components after processing, such as isopentanol, 2-methylbutanal, and isovaleraldehyde, were screened out.

Conclusion After processing, both the types and quantities of volatile components in Cuscuta chinensis significantly increased. The combination of electronic nose with GC-MS method can be used to identify the differences in volatile components among different processed products of Cuscuta chinensis, providing references for subsequent quality standards and clinical applications.

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