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Home Articles Vol 30,2026 No.6 Detail

Determination of three components in lysine vitamin B12 granules based on two-dimensional HPLC-MS

Published on Jul. 08, 2026Total Views: 63 times Total Downloads: 20 times Download Mobile

Author: WANG Xiaohui 1 LIU Yiqing 2 YANG Chun 1 GOU Qiongxin 1 SHAN Rumeng 2 WU Qi 2

Affiliation: 1.Lianyungang College of Traditional Chinese Medicine, Jiangsu Joint Vocational and Technical College, Lianyungang 222000, Jiangsu Province, China 2.Lianyungang Food and Drug Inspection and Testing Center, Lianyungang 222069, Jiangsu Province, China

Keywords: Two-dimensional high-performance liquid chromatography-triple quadrupole mass spectrometry method Vitamin B12 Lysine hydrochloride Inositol Content determination

DOI: 10.12173/j.issn.2097-4922.202604045

Reference: WANG Xiaohui, LIU Yiqing, YANG Chun, GOU Qiongxin1, SHAN Rumeng, WU Qi.Determination of three components in lysine vitamin B12 granules based on two-dimensional HPLC-MS[J]. Yaoxue QianYan Zazhi, 2026, 30(5): 996 - 1000.DOI: 10.12173/j.issn.2097-4922.202604045[Article in Chinese]

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Abstract

Objective To establish a two-dimensional high-performance liquid chromatography-triple quadrupole mass spectrometry method for the determination of three components in lysine and vitamin B12 granules.

Methods The one-dimensional chromatography used a mobile phase consisting of 0.005 mol/L potassium dihydrogen phosphate solution (adjusted to pH 5.5 with potassium hydroxide) and acetonitrile (92∶8) for the separation and trapping. The two-dimensional chromatography employed gradient elution using 0.1% formic acid solution as mobile phase A and 0.1% formic acid-methanol solution as mobile phase B. Electrospray ionization (ESI) was used, and the target compounds were qualitatively and quantitatively analyzed in positive-ion multiple reaction monitoring mode.

Results Lysine hydrochloride, vitamin B12, and inositol showed good linear relationships in the ranges of 80.06-8.006×106 μg/L, 10.12-1.012×103 μg/L, and 80.16-8.016×106 μg/L-1, respectively, with r values of 0.999 9. The average recovery rates were 98.71%, 97.96%, and 98.55%, respectively, with RSDs of 1.4%, 1.3%, and 0.9% (n=9).

Conclusion The method is simple in pretreatment, compatible with saline mobile phases, specific, sensitive, and capable of simultaneous quantitative analysis of the three components in lysine vitamin B12 granules.

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References

1. 国家药典委员会. 国家药品标准化学药品地标升国标(第十五五册)[S]. 2003: 16.

2. 耿颖, 黄慧芬, 魏宁漪, 等. HPLC法同时测定复合维生素B注射液中4种成分的含量[J]. 中国新药杂志, 2025, 34(15): 1647-1653.GengY, HuangHF, WeiNY, et al. Simultaneous determination of four components in compound vitamin B injection by HPLC[J]. Chinese Journal of New Drugs, 2025, 34(15): 1647-1653. DOI: 10.20251/j.cnki.1003-3734.2025.15.011.

3. 朱丹丹, 叶丽芳, 董宏然. HPLC法测定多种B族维生素微泡颗粒中维生素B12的含量[J]. 中国食品添加剂, 2025, 36(5): 125-130.ZhuDD,YeLF,DongHR.Determination of vitamin B12 content in various B vitamin microbubble particles by HPLC method[J]. Chinese Food Additives, 2025, 36(5): 125-130. DOI: 10.19804/j.issn1006-2513.2025.5.017.

4. 刘兆宏, 花锦. HPLC-DAD法测定草鱼肉质中的维生素B1和维生素B12的含量[J]. 中国饲料, 2024, (20): 79-83.LiuZH, H J. HPLC-DAD method for determining the content of vitamin B1 and vitamin B12 in grass carp meat[J]. China Feed, 2024, (20): 79-83. DOI:10.15906/j.cnki.cn11-2975/s.20242020.

5. 钱忠义, 葛薇薇, 仇雅静, 等. 高效液相-电喷雾检测法测定赖氨肌醇维B12口服溶液中肌醇的含量[J]. 中国药物警戒, 2022, 19(9): 959-961. [QianZY, GeWW, QiuYJ, et al. Determination of inositol in lysine inositol and vitamin B12 oral solution by high performance liquid chromatography electrospray detection[J]. China Pharmacovigilance, 2022, 19(9): 959-961.].https://d.wanfangdata.com.cn/periodical/ChdQZXJpb2RpY2FsQ0hJU29scjlRdWljaxIP emd5d2pqMjAyMjA5MDA2GghpMjh3aXRrZg%3D%3D.

6. 蓝燕玲, 潘云山, 温才洁, 等. 气相色谱法测定维生素预混料中肌醇的含量[J]. 山东化工, 2024, 53(17): 177-180.LanYL, PanYS, WenCJ, et al. Determination of inositol content in vitamin premixes by gas chromatography[J]. Shandong Chemical Industry, 2024, 53 (17): 177-180. DOI: 10.19319/j.cnki.issn.1008-021x.2024.17.018.

7. 刘文博. 气相色谱法测定保健食品中肌醇含量的方法研究[J]. 食品安全导刊, 2022, 16: 65-67. [LiuWB.Research on the method of determining inositol content in health food by gas chromatography[J]. Food Safety Guide,2022,16:65-67.DOI:10.16043/j.cnki.cfs.2022.16.022.

8. 舒森, 黄妮, 周军, 等. 柱前衍生-HPLC法测定葡萄糖酸钙锌口服溶液中盐酸赖氨酸含量[J]. 生物化工, 2025, 11(5): 128-131.ShuS, HuangN, ZhouJ, et al. Determination of lysine hydrochloride content in calcium zinc gluconate oral solution by pre column derivative HPLC method[J]. Biochemistry, 2025, 11(5): 128-131. DOI: 10.20284/j.cnki.swhg.2025.05.022.

9. 李昭, 李昌亮, 石笑弋, 等. 复方赖氨酸颗粒质量标准研究及改进[J]. 中国药品标准, 2024, 25(6): 555-559.LiZ, LiCL, ShiXY, et al. Research and improvement of quality standards for compound lysine granules[J]. Chinese Pharmaceutical Standards, 2024, 25(6): 555-559. DOI: 10.19778/j.chp.2024.06.004.

10. 何虹, 刘庄蔚, 蒋洁, 等. 不同方法测定口服五维赖氨酸葡萄糖中盐酸赖氨酸含量的研究[J]. 化学工程师, 2024, 38(5): 20-25. [HeH, LiuZW, JiangJ,et al. Study on the determination of lysine hydrochloride content in oral pentavitamin lysine glucose by different methods[J]. Chemical Engineer, 2024, 38(5):20-25.DOI: 10.16247/j.cnki.23-1171/tq.20240520.

11. 刘益庆, 陈洪岩, 杨晖, 等. 二维高效液相色谱-质谱联用检测化妆品中乌洛托品等4种禁用物质的方法[J]. 香料香精化妆品, 2025, (2): 106-110.LiuYQ, ChenHY, YangH, et al. A method for the determination of four prohibited substances in cosmetics, including urotropine, by two-dimensional high performance liquid chromatography-mass spectrometry[J]. Flavor essence cosmetics, 2025, (2): 106-110. DOI: 10.20099/j.issn.1000-4475.2024.0110.

12. 江舸, 郭志渊, 刘峰, 等. HPLC法和二维液相色谱-质谱联用法研究小儿复方氨基酸注射液中色氨酸有关物质[J]. 药学学报, 2026, 61(2): 553-559.Jiang G, Guo ZY, Liu F, et al Study on tryptophan related substances in pediatric compound amino acid injection by HPLC and two-dimensional liquid chromatography-mass spectrometry[J]. Acta Pharmaceutica Sinica, 2026, 61(2): 553-559. DOI: 10.16438/j.0513-4870.2025-1016.

13. 金雅文, 朱琼, 郑忠辉, 等. 盐酸伊伐布雷定有关物质的二维液相色谱-质谱联用分析[J]. 药物分析杂志, 2025, 45(10): 1816-1825.JinYW, ZhuQ, ZhengZH, et al. Two dimensional liquid chromatography-mass spectrometry analysis of related substances in ivabradine hydrochloride[J]. Journal of Pharmaceutical Analysis, 2025, 45(10): 1816-1825. DOI: 10.16155/j.0254-1793.2024-1319.

14. 祝福利, 杨红亮, 左为东, 等. 液相色谱-质谱法检测动物源性食品中β-受体激动剂残留研究进展[J]. 食品安全质量检测学报, 2025, 16(23): 111-121.ZhuFL, YangHL, ZuoWD, et al.Research progress on detection of β-receptor agonist residues in animal derived food by liquid chromatography-mass spectrometry[J]. Journal of Food Safety and Quality Inspection, 2025,16(23): 111-121. DOI:10.19812/j.cnki.jfsq11-5956/ts.20250806003.

15. 耿悦, 田壮壮, 陈民辉, 等. 现代质谱技术在头孢类抗生素杂质谱研究中的应用[J]. 药学与临床研究, 2025, 33(4): 351-356.GengY, TianZZ, ChenMH, et al.Application of modern mass spectrometry technology in the study of impurity spectra of cephalosporin antibiotics[J]. Pharmaceutical and Clinical Research, 2025, 33(4): 351-356. DOI: 10.13664/j.cnki.pcr.2025.04.009.

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