Study on the separation, purification and hematopoietic activity of Pelodiscus sinensis peptides

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Author: LI Yizhen ¹ LIU Yanxin ¹ ZHANG Jinhua ¹  LIU Fei ¹ CHEN Lei ¹ DIAO Mengqi ¹ ZHANG Linjun ¹ ZHANG Yanyan ¹

Affiliation: 1.Shandong Engineering Research Center for Efficient Preparation and Application of Sugar and Sugar Complex, Shandong Provincial Key Laboratory of Sugar and Sugar Complex Drugs, Shandong Academy of Pharmaceutical Science, Jinan 250101, China

Keywords: Pelodiscus sinensis peptides Separation Purification Hematopoietic activity

DOI: 10.12173/j.issn.2097-4922.202601034

Reference: LI Yizhen, LIU Yanxin, ZHANG Jinhua, LIU Fei, CHEN Lei, DIAO Mengqi, ZHANG Linjun, ZHANG Yanyan.Study on the separation, purification and hematopoietic activity of Pelodiscus sinensis peptides[J]. Yaoxue QianYan Zazhi, 2026, 30(5): 951 - 956.DOI: 10.12173/j.issn.2097-4922.202601034[Article in Chinese]  Copied

Abstract
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Abstract

Objective To study the separation, purification and hematopoietic activity of Pelodiscus sinensis peptides.

Methods The Pelodiscus sinensis peptides were prepared by enzymatic hydrolysis and purified using ÄKTA protein purification system. The moisture content was measured using a moisture analyzer, and the total peptide content was detected using the bicinchoninic acid assay method. The molecular weight distribution was analyzed by gel permeation chromatography, and its hematopoietic activity was evaluated by zebrafish model.

Results After separation and purification, 5 turtle peptide components (PPSP1, PPSP2, PPSP3, PPSP4, and PPSP5) were obtained. The yield of PPSP3 was the highest (34.76%); PPSP2 and PPSP4 had relatively low moisture contents at 5.03% and 4.95%, respectively; PPSP1 and PPSP3 had relatively high total peptide contents at 90.2% and 87.4%, respectively; PPSP3 and PPSP4 had the highest proportion of small molecular peptieds (< 1 000 Da), accounting for 92.94% and 93.11%, respectively. All 5 components had hematopoietic activity, with PPSP5 had significantly better hematopoietic activity than other components.

Conclusion Although the 5 components of soft-shelled turtle peptides obtained through separation and purification have different physical and chemical properties, they all exhibit hematopoietic activity, with PPSP5 showing particularly significant activity.

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