Study on the inspection method ofLonicerae flos in Jinju Wuhuacha granules

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Author: PAN Zhiwen ¹ GUO Haibin ¹  CHEN Yihua ¹

Affiliation: 1.Foshan Food and Drug Inspection and Testing Center,Foshan528051,Guangdong Province,China

Keywords: Jinju Wuhuacha granules Lonicerae Flos Macranthoidin B UPLC-MS/MS

DOI: 10.12173/j.issn.2097-4922.202606018

Abstract
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Abstract

Objective An ultra performance liquid chromatography tandem triple quadrupole mass spectrometry (UPLC-MS/MS) method was established to effectively identify the possible adulteration of Lonicerae Flos in Jinju Wuhuacha granules with macranthoidin B as the index component.

Methods The analysis was performed on a 30 ℃ thermostatic Waters BEH C₁₈ column (50 mm × 2.1 mm, 1.7 μm), with the mobile phase comprising of water-acetonitrile (both containing 0.1% formic acid) flowing at 0.3 mL/min in a gradient elution manner, and the injection volume was 2 μL, and electronspray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.

Results Macranthoidin B exhibited a good linear relationship within the concentration range of 0.10 to 10.00 μg/mL, with a correlation coefficient of 0.999 5. The recovery rate was 101.31%, and theRSD was 2.94% (n=6). Among the 30 batches of samples, macranthoidin B was detected in 15 batches, and the adulteration level of 4 batches exceeded 3%.

Conclusion The method is highly sensitive, specific, rapid and accurate, and can provide reference for the inspection of adulteratedLonicerae Flos in Jinju Wuhuacha granules.

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