Objective  To establish the benchmark fingerprint and multi-index component content determination methods for 15 batches of  Dayuan drink, study the rules of value transfer, and provide reference for the quality control and evaluation of the classic formula Dayuan drink.

Methods   15 batches of reference samples of Dayuan drink were prepared according to ancient research, and HPLC was used to establish the fingerprint and multi-component content determination method of Dayuan drink. The chromatographic peak attribution and similarity differences among batches were clarified. Through the content determination of indicative component, the transfer rules from medicinal herbs to reference substance values was understood, and a research system for the reference substance quality of Dayuan drink was established.

Results  The benchmark fingerprint spectra of 15 batches of Dayuan drink were calculated using similarity software with baicalin as the reference peak, and the similarity was >0.97. There were 24 common peaks, and 12 chromatographic peaks were identified based on the reference spectrum. The content determination showed that paeoniflorin, baicalin, and wogonoside were the higher content and had higher transfer rate, while the transfer rate of Magnolia officinalis was the lowest.

Conclusion  The fingerprint combined with multi-index component content determination method is suitable for quality control and evaluation of the substance benchmark of Dayuan drink. It is easy to operate and provides a scientific basis for the quality control and subsequent formulation development of Dayuan drink, combined with the overall value transfer rules.

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