Objective  To establish an analytical method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology for simultaneous screening of 9 veterinary drug residues in Xiaohuoluo pills.

Methods  The samples were extracted and purified using SHIMSEN QuEChERS tubes for vet drugs. Separation was performed using a Shim pack GIST C₁₈ column (100  mm×2.1 mm, 2 μm). The mobile phase was methanol-0.1% formic acid solution (containing 2 mmol/L ammonium acetate) and gradient elution was performed. The flow rate was 0.2 mL/min, and the column temperature was 40 ℃. The ionization used an electric spray ion source to conduct multi reaction monitoring in the positive and negative ion switching mode.

Results  The linear relationship between 9 veterinary drugs was good within the corresponding concentration range, with r values greater than 0.997 0. The detection limit of the method was 0.000  5-0.001 4  mg/ kg, and the quantification limit was 0.002-0.005 mg/kg. The average recovery rate was 76.47%-102.44%.

Conclusion  This method has high sensitivity, strong accuracy, and good applicability, and can be used for rapid screening and detection of related veterinary drug residues in Xiaohuoluo pills.

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