Objective  To optimize the water extraction and alcohol precipitation process of Radix Paeoniae Rubra-Cortex Moutan drug pair (1∶1).

Methods L9 (34) orthogonal experiment was used. The ratio of material to liquid, extraction time and times of extraction were taken as the factors, and the extraction amount of paeoniflorin and paeonol and the yield of dry extract were taken as the indexes. Hassan’s method was used for multi-index comprehensive scoring to optimize the water extraction process. The extract density, ethanol volume fraction and standing time were taken as the factors, and the transfer rate of paeoniflorin and paeonol and the yield of dry extract were taken as the indexes. At the same time, the alcohol precipitation process was optimized according to the actual situation of large-scale production.

Results  The optimal extraction process was to add 10 times of water and decoct for 1 time, 60 min for each extraction. The optimal alcohol precipitation process was as follows: the density of the liquid was 1.20 g·mL^-1 (30℃), 95% ethanol was added to make the alcohol content up to 60%, and the liquid was left standing for 60 h.

Conclusion  The optimized process of water extraction and alcohol precipitation is stable, reliable and with good repeatability, which can provide reference for the follow-up study of Radix Paeoniae Rubra- Cortex Moutan drug pair.

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