Objective  To established a new method for detecting catalase activity to address the issues of high detection limit, insufficient sensitivity, and poor stability of the colorimetric solution in the previous colorimetric method.

Methods  Fe²⁺ did not undergo color reaction with SCN-. However, when Fe²⁺ was oxidized to Fe³⁺ by H₂O₂, Fe³⁺ formed a Fe³⁺/SCN- complex with SCN- that exhibited maximum absorption at 458 nm. The absorbance was positively correlated with the concentration of H₂O₂. When catalase was present, it catalysed the decomposition of H₂O₂, resulting in a decreasing absorbance with time, and the rate of decrease (∆A/t) reflected the catalytic activity of the enzyme.

Results  The effective detection range for catalase activity was 20-400 U/L, with a minimum detection limit of 10 U/L.

Conclusion  This study is the first to use inorganic color reagents in photometry and construct an accurate, stable, and anti-interference method for measuring catalase activity through coordination reactions. This method is suitable for detecting trace enzyme activity and has good promotion and application value.

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