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Home Articles Vol 30,2026 No.3 Detail

Predicting the differential efficacy of vitiligo between Tripterygium wilfordii and Tripterygium hypoglaucum and screening for candidate key microRNAs based on their main triterpene components

Published on Apr. 02, 2026Total Views: 23 times Total Downloads: 4 times Download Mobile

Author: ZHAO Jiangyi 1 ZENG Qin 1 CAI Beilei 2 LIU Qiancheng 1 LI Yongjun 1 ZHAO Jianquan 1, 3 WANG  Jiangrui 1, 2

Affiliation: 1. Pharmaceutical Factory of Chongqing Academy of Chinese Materia Medica, Chongqing 400800, China 2. Sinomune Pharmaceutical Co., Ltd, Wuxi 214194, Jiangsu Province, China 3. Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China

Keywords: Tripterygium wilfordii Tripterygium hypoglaucum Network pharmacology Molecular docking Triterpene components Vitiligo Pharmacological differences microRNA

DOI: 10.12173/j.issn.2097-4922.202511014

Reference: ZHAO Jiangyi, ZENG Qin, CAI Beilei, LIU Qiancheng, LI Yongjun, ZHAO Jianquan, WANG  Jiangrui. Predicting the differential efficacy of vitiligo between Tripterygium wilfordii and Tripterygium hypoglaucum and screening for candidate key microRNAs based on their main triterpene components[J]. Yaoxue QianYan Zazhi, 2026, 30(3): 383-393. DOI: 10.12173/j.issn.2097-4922.202511014.[Article in Chinese]

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Abstract

Objective  To predict the differential efficacy of Tripterygium wilfordii and Trip-terygium hypoglaucum in improving vitiligo based on the main triterpenoid components, and to screen candidate key microRNAs for the action of triterpenoid components, laying a theoretical foundation for subsequent new drug development.

Methods  The content of the main triterpenoid active components (demethylzeylasteral, celastrol, and pristmerin) in Tripterygium wilfordii and Tripterygium hypoglaucum were determined using high with performance liquid chromatography-diode array detection (HPLC-DAD) method, combined with network pharmacology and molecular docking to predict the activity of triterpenoid components in treating vitiligo, and to comprehensively evaluate the efficacy of Tripterygium wilfordii and Tripterygium hypoglaucum in improving vitiligo, while predicting the candidate key microRNAs involved.

Results  The content of demethylzeylasteral and celastrol in Tripterygium wilfordii and the comprehensive efficacy score for improving vitiligo was significantly higher than those of Tripterygium hypoglaucum (P<0.01). At the same time, 17 candidate key microRNAs for the treatment of vitiligo by triterpenoid components, including hsa-miR-7-5p, hsa-miR-133a-5p, and hsa-miR-4795-3p, were screened.

Conclusion  Both Tripterygium wilfordii and Tripterygium hypoglaucum can regulate multiple microRNAs to improve the symptoms of vitiligo, but their efficacy differs significantly, with Tripterygium wilfordii being more suitable for the treatment of vitiligo compared to Tripterygium hypoglaucum.

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