Objective To establish characteristic chromatogram and target component content determination methods of Jingzhu Qingjie granules, as well as to improve quality standards.

Methods The ZORBAX Eclipse XDB-C₁₈ column (250 mm × 4.6 mm, 5 μm) was employed. The gradient elution was performed using a mobile phase of 0.1% phosphoric acid-acetonitrile solution. Detection was conducted at a wavelength of 280 nm, with a flow rate of 1.0 mL/min, a 10 µL injection volume, and a column temperature of 30 ℃. This method was used to establish the HPLC characteristic chromatogram of Jingzhu Qingjie granules. Moreover, similarity analysis, cluster analysis, and principal component analysis were applied to analyze 10 batches of samples. Base on the content determination, forsythoside A was detected 327 nm, while arctiin was detected at 280 nm. All other chromatographic conditions were consistent with the characteristic chromatogram. This method was used to determine the target component content in Jingzhu Qingjie granules.

Results The characteristic chromatogram was established for Jingzhu Qingjie granules, identifying eight characteristic peaks and confirming seven components. The similarity between the characteristic chromatograms of 10 batches of Jingzhu Qingjie Granules and its reference chromatogram was consistently greater than 0.85. The two chemometric analyses classified the samples into three major categories. The content of forsythoside A across 10 batches of Jingzhu Qingjie granules was 1.30-1.94 mg/g, while the arctiin content was 3.00-9.35 mg/g.

Conclusion The established characteristic chromatogram and content determination methods for Jingzhu Qingjie granules are stable and feasible, providing data support for their quality control.

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