Pharmacognostical identification research of Inonotus obliquus

Published on Jul. 31, 2024Total Views: 1171 times Total Downloads: 310 times Download Mobile

Author: ZHU Tianmi ¹ DUAN Yuqing ² WANG Simeng ² CHEN Shuhe ¹ YANG Ying ³  CHEN Keli ²

Affiliation: 1. Department of Pharmacy, Hubei Provincial Hospital of Traditional Chinese Medicine/Affiliated Hospital of Hubei University of Chinese Medicine/Hubei Institute of Traditional Chinese Medicine, Wuhan 430061, China 2. School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China 3. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

Keywords: Inonotus obliquus Medicinal parts Basal source investigation Trait identification Microscopic identification Molecular biology High-performance liquid chromatography Content determination

DOI: 10.12173/j.issn.1008-049X.202312062

Reference: ZHU Tianmi, DUAN Yuqing, WANG Simeng, CHEN Shuhe, YANG Ying, CHEN Keli.Pharmacognostical identification research of Inonotus obliquus[J].Zhongguo Yaoshi Zazhi,2024, 27(7):1108-1114.DOI: 10.12173/j.issn.1008-049X.202312062.[Article in Chinese]  Copied

Abstract
Full-text
References

Abstract

Objective To study the identification methods and traits of the Chinese medicinal materials Inonotus obliquus.

Methods Samples were collected from the origin of northeast China, and source identification, molecular biology identification, trait identification, microscopic identification and HPLC assay were used to study the Inonotus obliquus, its non-pure fungous part, and counterfeit.

Results Inonotus obliquus had a nodular shape, the microstructure was closely interconnected with mycelia, and the medicinal part was the sclerotium, not the “fruiting body” as stipulated in the current standards of traditional Chinese medicinal materials. Occasionally, a very small amount of tubule and subiculum could be seen in Inonotus obliquus. Tubule hole shape was circular, with a diameter of 130-190 μm, and ruptured. The pipe wall structure was special, which was composed of longitudinal arranged small compartment with diameter of 2-5-8 μm. Non-pure fungous part was the decayed host xylem infiltrated by mycelia. The contents of trametenolic acid and ergosterol in this part were significantly lower than the pure sclerotium (P<0.05). The microstructure and HPLC fingerprints of Inonotus obliquus and the counterfeit burl were clearly distinguishable from each other.

Conclusion The method used is able to identify the authenticity of Inonotus obliquus and the quality of the medicinal materials.

Full-text

Please download the PDF version to read the full text: download

References

1.张小青, 戴玉成, 编著. 中国真菌志第二十九卷锈革孔菌科. 第1版[M]. 北京: 科学出版社, 2005: 92-93.

2.李玉, 李泰辉, 杨祝良, 等, 编著. 中国大型菌物资源图鉴. 第1版[M]. 郑州: 中原农民出版社, 2015: 474-475.

3.黄年来. 俄罗斯神秘的民间药用真菌——桦褐孔菌[J].中国食用菌, 2002, 21(4): 7-8. [Huang NL. Mysterious folk medicinal fungi in Russia——Inonotus obliquus[J]. Edible Fungi of China, 2002, 21(4): 7-8.] DOI: 10.13629/j.cnki.53-1054.2002.04.002.

4.Duru KC, Kovaleva EG, Danilova IG, et al. The pharmacological potential and possible molecular mechanisms of action of Inonotus obliquus from preclinical studies[J]. Phytother Res, 2019, 33(8): 1966-1980. DOI: 10.1002/ptr.6384.

5.Zhao YX, Zheng WF. Deciphering the antitumoral potential of the bioactive metabolites from medicinal mushroom Inonotus obliquus[J]. J Ethnopharmacol, 2021, 265: 113321. DOI: 10.1016/j.jep.2020.113321.

6.马传贵, 张志秀, 肖宝, 等. 桦褐孔菌的生物活性及其应用研究[J]. 食用菌, 2022, 44(2): 1-4. [Ma CG, Zhang ZX, Xiao B, et al. Biological activity and application research of Inonotus obliquus[J]. Acta Edulis Fungi, 2022, 44(2): 1-4.] DOI: 10.3969/j.issn.1000-8357.2022.02.001.

7.崔贺铭, 高洋, 张玉莹, 等. 不同厂家的桦褐孔菌保健品中的活性成分含量分析[J]. 长春中医药大学学报, 2022, 38(5): 510-512. [Cui HM, Gao Y, Zhang YY, et al. Analysis of active ingredients in health products of Inonotus obliquus from different manufacturers[J]. Journal of Changchun University of Chinese Medicine, 2022, 38(5): 210-512.] DOI: 10.13463/j.cnki.cczyy.2022.05.011.

8.山东省食品药品监督管理局. 山东省中药材标准[S]. 2013: 232-235.

9.湖北省药品监督管理局. 湖北省中药材质量标准[S]. 2019: 182-183.

10.刘国涛, 高攀攀, 魏凯. 药食两用真菌—桦褐孔菌[J]. 山东畜牧兽医, 2021, 42(7): 65-66. [Liu GT, Gao PP, Wei K. Medicinal and edible fungi—Inonotus obliquus[J]. Shandong Journal of Animal Science and Veterinary Medicine, 2021, 42(7): 65-66.] DOI: 10.3969/j.issn.1007- 1733.2021.07.026.

11.李艳婷, 郭尚, 徐莉娜, 等. 桦褐孔菌资源分布及其地域环境条件分析[J]. 中国林副特产, 2019, (4): 60-65. [Li YT, Guo S, Xu LA, et al. Study on review of the distribution and environmental conditions of Inonotus obliquus[J]. Forest By-Product and Speciality in China, 2019, (4): 60-65.] DOI: 10.13268/j.cnki.fbsic.2019.04.024.

12.陈正启, 周汐, 华蓉, 等. 桦褐孔菌的研究现状及应用前景[J]. 中国食用菌, 2021, 40(10): 1-6. [Chen ZQ, Zhou X, Hua R, et al. Research status and application prospects of Inonotus obliquus[J]. Edible Fungi of China, 2021, 40(10): 1-6.] DOI: 10.13629/j.cnki.53-1054.2021.10.001.

13.朱洪庆, 王盼盼, 张萌, 等. rDNA-ITS序列分析法与传统分类法相结合在真菌鉴定中的应用[J]. 西华师范大学学报(自然科学版)，2016，37(3): 264-269. [Zhu HQ, Wang PP, Zhang M, et al. Application of rDNA-ITS sequence analysis and traditional classification method in the identification of fungi[J]. Journal of China West Normal University (Natural Sciences), 2016, 37(3): 264-269.] DOI: 10.16246/j.issn.1673-5072.2016.03.006.

14.Stefanova P, Brazkova M, Angelova G. Comparative study of DNA extraction methods for identification of medicinal mushrooms[J]. BIO Web Conf, 2022, 45: 02007. DOI: 10.1051/bioconf/20224502007.

15.朱田密, 陈树和, 陈科力. 8份灵芝习用品的生药学鉴别[J]. 华西药学杂志, 2021, 36(6): 714-716. [Zhu TM, Chen SH, Chen KL. Pharmacognosy identification of eight relative byproducts of Ganoderma[J]. West China Journal of Pharmaceutical Sciences, 2021, 36(6): 714-716.] DOI: 10.13375/j.cnki.wcjps.2021.06.026.

16.朱田密, 柳阳, 陈树和. 树舌药材的质量标准研究 [J]. 亚太传统医药, 2021, 17(4): 47-49. [Zhu TM, Liu Y, Chen SH. Study on the quality standard of Ganoderma applanatum[J]. Asia-Pacific Traditional Medicine, 2021, 17(4): 47-49.] DOI: 10.11954/ytctyy.202104011.

17.江泽慧, 彭镇华, 著. 世界主要树种木材科学特性. 第2版 [M]. 北京: 科学出版社, 2016: 42.

18.段雨晴, 朱田密, 陈树和, 等. 高效液相色谱法测定桦褐孔菌4种成分含量及不同部位含量比较[J]. 医药导报, 2023, 42(3): 339-345. [Duan YQ, Zhu TM, Chen SH, et al. Determination of four components of Inonotus obliquus by HPLC and comparison of contents in different parts[J]. Herald of Medicine, 2023, 42(3): 339-345.] DOI: 10.3870/j.issn.1004-0781.2023.03.009.

19.段雨晴, 朱田密, 陈树和, 等. 桦褐孔菌药材的薄层色谱鉴别和HPLC指纹图谱建立及化学模式识别分析[J]. 中国药房, 2023, 34(1): 52-56. [Duan YQ, Zhu TM, Chen SH, et al. Establishment of TLC identification and HPLC fingerprint of Inonotus obliquus and analysis of chemical pattern recognition[J]. China Pharmacy, 2023, 34(1): 52-56.] DOI: 10.6039/j.issn.1001-0408.2023.01.10.