Rapid identification of chemical constituents in Yaokangning decoction by UPLC-Q-TOF-MS/MS

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Author: Qian ZHANG ¹ Pei ZHANG ¹ Hai-Feng LI ¹ Le-Jing JIANG ¹ Zeng-Min YANG ²  Hui JI ²

Affiliation: 1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China 2. Second Department of Orthopedics, Nanjing Integrated Traditional Chinese and Western Medicine Hospital Affiliated with Nanjing University of Chinese Medicine, Nanjing 210014, China

Keywords: UPLC-Q-TOF-MS/MS Yaokangning decoction Flavonoids Organic acids Monoterpene glycosides

DOI: 10.12173/j.issn.1008-049X.202210027

Reference: Qian ZHANG, Pei ZHANG, Hai-Feng LI, Le-Jing JIANG, Zeng-Min YANG, Hui JI.Rapid identification of chemical constituents in Yaokangning decoction by UPLC -Q-TOF-MS/MS[J].Zhongguo Yaoshi Zazhi,2023,26(10):150-155.DOI: 10.12173/j.issn.1008-049X.2[Article in Chinese]  Copied

Abstract
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Abstract

Objective To rapidly identify the chemical constituents in Yaokangning decoction by ultra performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS).

Methods The sample was performed on a ACQUITY UPLC BEH C₁₈ column (150 mm×2.1 mm, 1.7 μm) with 0.2% formic acid water methanol as the mobile phase system by gradient elution. The injection volume was 3 μL, the column temperature was 40℃, and the volume flow rate was 0.5 mL·min^-1. The chromatographic effluents were detected by electrospray ionization in positive and negative ion mode.

Results A total of 10 chemical components were identified by comparing with reference substances and combining with literature, including kaempferitrin, baohuoside I, puerarin, calycosin-7-O-β-D-glucoside, paeoniflorm, schaftoside, ononin, salvianolic acid B, octacosanoic acid, and 4H-1-benzopyran-2-carboxylicac.

Conclusion The established method can systematically and quickly analyze the chemical constituents of Yaokangning decoction, which could provide an important basis for the quality control and pharmacodynamic material basis study of Yaokangning decoction.

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