Objective  To investigate whether curdione inhibits the formation of deep vein thrombosis (DVT) in rats through thrombolytic effects.

Methods  Twenty-five rats were randomly divided into five groups, the normal control group, the sham operation group, the model group, the low dose group of curdione (60 mg/kg) and the high dose group of curdione (120 mg/kg), with five rats in each group. Using the inferior vena cava ligation model, blood was taken from the pulmonary aorta in a sodium citrate anticoagulant tube one week after drug administration. The coagulation and fibrinolytic indexes Fibrin Degradation Products, (FDP), D-dimer (D-D), plasminogen activator inhibitor (PAI)-1 and plasminogen (PLG) were detected in each group; the thrombus weight/weight-length ratio was detected; and the formation of venous thrombus in the lower limbs of rats was detected by hematoxylin-eosin (HE) staining nuclear factor; NF-κB protein expression in inferior vena cava tissues was dectected by Western Blot assay.

Results  There was no significant difference in plasma FDP levels among groups of rats (P﹥0.05); compared with the model group, the plasma FDP and D-D levels of rats in each dosing group were increased, the plasma PAI-1 and PLG levels were elevated, and the fibrinolytic effect was better in the group with high dose of curdione administration than in the group with low dose of curdione administration (P ﹤0.05); compared with the model group, the thrombus weight/weight-length ratio, the thrombus mass and NF-κB protein expression in the curdione dosing group were decreased.

Conclusion  Curdione can inhibit the formation of DVT in rats by influencing the fibrinolytic regulators.

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