Ficus carica L. is a medicinal and edible plant with a long history of use. It has the effects of clearing heat and producing fluids, strengthening the spleen and improving appetite, detoxifying and detumescence, moistening the lungs and relieving cough. Modern studies have shown that Ficus carica L. mainly contains chemical components such as polyphenols, phenylpropanoids, polysaccharides, triterpenoids, sterols, volatile components, fatty acids. Pharmacological studies have confirmed that it mainly has antitumor, regulating lipid metabolism, hypoglycemic activity, liver and kidney protection, anti-inflammatory, immune regulation, antibacterial, neuroprotective, treatment of skin diseases, cardiovascular and cerebrovascular protection, and other effects. This paper summarizes recent research reports on the chemical constituents and pharmacological effects of Ficus carica L. at home and abroad, to provide reference for the in-depth excavation of the medicinal value and the development of related products of Ficus carica L.
1.徐新春, 吴明光. 无花果本草考证[J]. 中国中药杂志, 2001, 26(6): 392-393. [Xu XC, Wu MG. A study on the medicinal properties of figs[J]. China Journal of Chinese Materia Medica, 2001, 26(6): 392-393.] DOI: 10.3321/ j.issn:1001-5302.2001.06.010.
2.姜宏伟, 李春英, 赵春建, 等. 无花果的化学成分、药理作用和临床应用[J]. 黑龙江科学, 2019, 10(6): 12-15. [Jiang HW, Li CY, Zhao CJ, et al. The chemical composition, pharmacological activity and clinical application of Ficus carica L.[J]. Heilongjiang Science, 2019, 10(6): 12-15.] DOI: 10.3969/j.issn.1674-8646.2019.06.004.
3.江苏新医学院,编. 中药大辞典[M]. 上海: 上海人民出版社, 1977: 341.
4.Kebal L, Pokajewicz K, Djebli N, et al. HPLC-DAD profile of phenolic compounds and in vitro antioxidant activity of Ficus carica L. fruits from two Algerian varieties[J]. Biomed Pharmacother, 2022, 155: 113738. DOI: 10.1016/j.biopha.2022.113738.
5.迪丽阿热姆·尼加提. 无花果叶汁的化学成分研究[D]. 乌鲁木齐: 新疆医科大学, 2016. DOI: 10.7666/d.D834789.
6.Cruz JMDA, Corrêa RF, Lamarão CV, et al. Ficus spp. fruits: bioactive compounds and chemical, biological and pharmacological properties[J]. Food Res Int, 2022, 152: 110928. DOI: 10.1016/j.foodres.2021.110928.
7.丁真真, 夏娜, 刘艳全, 等. 不同干燥方式对5种无花果干营养成分、抗氧化活性和挥发性成分的影响[J]. 食品工业科技, 2025, 46(13): 292-302. [Ding ZZ, Xia N, Liu YQ, et al. Effects of different drying methods on nutritional components,antioxidant activity and volatile components of five kinds of dried figs[J].Science and Technology of Food Industry, 2025, 46(13): 292-302.] DOI: 10.13386/j.issn1002-0306.2024080042.
8.夏婷, 赵超亚, 杜鹏, 等. 食品中多酚类化合物种类、提取方法和检测技术研究进展[J]. 食品与发酵工业, 2019, 45(5): 231-238. [Xia T, Zhao CY, Du P, et al. Research progress on classification, extraction, and detection of polyphenols in foods[J]. Food and Fermentation Industries, 2019, 45(5): 231-238.] DOI: 10.13995/j.cnki.11-1802/ts.017498.
9.Ghouizi AE, Ousaaid D, Laaroussi H, et al. Ficus carica (Linn.) leaf and bud extracts and their combination attenuates type-1 diabetes and its complications via the inhibition of oxidative stress[J]. Foods, 2023, 12(4): 759. DOI: 10.3390/foods12040759.
10.胡雪梅, 李金平, 游勇, 等. 无花果全株化学成分及药用价值研究进展[J]. 四川农业科技, 2023, (3): 103-106. [Hu XM, Li JP, You Y, et al. Research progress on chemical constituents and medicinal value of the whole fig plant[J]. Sichuan Agricultural Science and Technology, 2023, (3): 103-106.] DOI: 10.3969/j.issn.1004-1028.2023.03.029.
11.董丽梅, 孔令熙, 陈玳欣, 等. 无花果中黄酮类化学成分及抗氧化活性研究[J]. 食品安全导刊, 2023, (34): 93-95, 99. [Dong LM, Kong LX, Chen DX, et al. Study on chemical constituents and antioxidant activities of flavonoids in fig[J]. China Food Safety Magazine, 2023, (34): 93-95, 99.] DOI: 10.16043/j.cnki.cfs.2023.34.042.
12.刘鹏莉, 陈英乡, 遇艳萍, 等. 无花果营养成分及生物活性研究进展[J]. 食品工业科技, 2023, 44(6): 424-431. [Liu PL, Chen YX, Yu YP, et al. A review of nutritional components and biological activity of Ficus carica[J]. Science and Technology of Food Industry, 2023, 44(6): 424-431.] DOI: 10.13386/j.issn1002-0306.2022050002.
13.Li Z, Yang Y, Liu M, et al. A comprehensive review on phytochemistry, bioactivities, toxicity studies, and clinical studies on Ficus carica Linn. leaves[J]. Biomed Pharmacother, 2021, 137: 111393. DOI: 10.1016/j.biopha.2021.111393.
14.吴子阳, 何菲, 李贺贺, 等. 采用固相萃取结合高效液相色谱测定清香型白酒中酚类活性化合物[J]. 分析化学, 2020, 48(10): 1400-1408. [Wu ZY, He F, Li HH, et al. Determination of phenolic active compounds in light-aroma Baijiu by solid phase extraction-high performance liquid chromatography[J]. Chinese Journal of Analytical Chemistry, 2020, 48(10): 1400-1408.] DOI: 10.19756/j.issn.0253-3820.191565.
15.Liu YP, Guo JM, Yang G, et al. Anti-inflammatory and antiproliferative prenylated isoflavone derivatives from the fruits of Ficus carica[J]. J Agric Food Chem, 2019, 67(17): 4817-4823. DOI: 10.1021/acs.jafc.9b00865.
16.邵明辉, 张文浩, 张明明, 等. 无花果果实中化学成分研究 [J]. 中草药, 2019, 50(11): 2524-2528. [Shao MH, Zhang WH, Zhang MM, et al. Chemical constituents from fruits of Ficus caric[J]. Chinese Traditional and Herbal Drugs, 2019, 50(11): 2524-2528.] DOI: 10.7501/j.issn.0253-2670.2019.11.003.
17.Ammar S, Contreras MM, Belguith-Hadrich OS, et al. Assessment of the distribution of phenolic compounds and contribution to the antioxidant activity in Tunisian fig leaves, fruits, skins and pulps using mass spectrometry-based analysis[J]. Food Funct, 2015, 6(12): 3663-3677. DOI: 10.1039/c5fo00837a.
18.李铭柔. 无花果根中化学成分及生物活性的研究[D]. 西安: 西北大学, 2022. DOI: 10.27405/d.cnki.gxbdu.2022.002044.
19.Aloud AA, Chinnaduraib V, Chandramohan G, et al. Galangin controls streptozotocin-caused glucose homeostasis and reverses glycolytic and gluconeogenic enzyme changes in rats[J]. Arch Physiol Biochem, 2018, 126(2): 101-106. DOI: 10.1080/13813455.2018.1498521.
20.Rehman K, Chohan TA, Waheed I, et al. Taxifolin prevents postprandial hyperglycemia by regulating the activity of α‐amylase: evidence from an in vivo and in silico studies[J]. J Cell Biochem, 2018, 120(1): 425-438. DOI: 10.1002/jcb.27398.
21.Chen YJ, Kong L, Tang ZZ, et al. Hesperetin ameliorates diabetic nephropathy in rats by activating Nrf2/ARE/glyoxalase 1 pathway[J]. Biomed Pharmacother, 2019, 111: 1166-1175. DOI: 10.1016/j.biopha.2019.01.030.
22.Turkoglu M, Pekmezci E, Kilic S, et al. Effect of Ficus carica leaf extract on the gene expression of selected factors in HaCaT cells[J].J Cosmet Dermatol, 2017, 16(4): 54-58. DOI: 10.1111/jocd.12344.
23.Li B, Li Z, Tan Y, et al. Latex derived from Ficus carica L. inhibited the growth of NSCLC by regulating the caspase/gasdermin/AKT signaling pathway[J]. Food Funct, 2023, 14(4): 2239-2248. DOI: 10.1039/d2fo02284b.
24.李明, 安熙强, 马媛, 等. 无花果研究进展[J]. 新疆中医药, 2010, 28(1): 79-80. [Li M, An XQ, Ma Y, et al. Research progress on fig[J]. Xinjiang Journal of Traditional Chinese Medicine, 2010, 28(1): 79-80.] DOI: 10.3969/j.issn.1009-3931.2010.01.040.
25.聂菲, 宛燕, 曾杰, 等. HS-SPME-GC-MS法测定不同发育期无花果果实的挥发性成分[J]. 现代食品, 2024, 30(17): 197-202. [Nie F, Wan Y, Zeng J, et al. Determination of volatile compounds of Ficus carica L. fruits at different developing stages with HS-SPME-GC-MSL[J]. Modern Food, 2024, 30(17): 197-202.] DOI: 10.16736/j.cnki.cn41-1434/ts.2024.17.047.
26.刘志杰, 张薇. 基于网络药理学探讨无花果治疗原发性肝癌的作用机制[J]. 广东化工, 2024, 51(1): 123, 130-132. [Liu ZJ, Zhang W. Exploring the mechanism of action of figs in the treatment of primary hepatocellular carcinoma based on network pharmacology[J]. Guangdong Chemical Industry, 2024, 51(1): 123, 130-132.] DOI: 10.3969/j.issn.1007-1865.2024.01.040.
27.庄奕筠, 张吟. 无花果叶的药用研究进展[J].海峡药学, 2011, 23(12): 1-4. [Zhuang YY, Zhang Y. Medical research progress on Ficus carica leaves[J]. Strait Pharmaceutical Journal, 2011, 23(12): 1-4.] DOI: 10.3969/j.issn.1006-3765.2011.12.001.
28.Ye L, Zhang QQ, Lin S, et al. A Polysaccharide from Ficus carica L. exerts immunomodulatory activity in both in vitro and in vivo experimental models[J]. Foods, 2024, 13(2): 195. DOI: 10.3390/foods13020195.
29.Du J, Li J, Zhu J, et al. Structural characterization and immunomodulatory activity of a novel polysaccharide from Ficus carica[J]. Food Funct, 2018, 9(7): 3930-3943. DOI: 10.1039/c8fo00603b.
30.张卫平, 李新生, 曲东, 等. 无花果主要功能成分及其生物活性研究进展[J]. 食品研究与开发, 2023, 44(20): 212-218. [Zhang WP, Li XS, Qu D, et al. Research progress on main functional components and bioactivity of Ficus carica L.[J]. Food Research and Development, 2023, 44(20): 212-218.] DOI: 10.12161/j.issn.1005-6521.2023.20.029.
31.Zhang T, Chen M, Li D, et al. Review of the recent advances in polysaccharides from Ficus carica: extraction, purification, structural characteristics, bioactivities and potential applications[J]. Int J Biol Macromol, 2024, 281: 136430. DOI: 10.1016/j.ijbiomac.2024.136430.
32.周春华, 陶俊, 韦军, 等. 无花果化学成分和生物活性研究进展[A]. 中国园艺学会第八届青年学术讨论会暨现代园艺论坛论文集[C]. 上海: 中国园艺学会, 2008.
33.谢建华, 郭小妹, 袁兰兰, 等. 甾醇类化合物降低高胆固醇HepG2细胞内胆固醇的作用机制[J]. 食品科学, 2023, 44(5): 68-74. [Xie JH, Guo XM, Yuan LL, et al. Mechanism of action of sterols in reducing intracellular cholesterol in hypercholesterolemic HepG2 cells[J]. Food Science, 2023, 44(5): 68-74.] DOI: 10.7506/spkx1002-6630-20211105-071.
34.李金玉. GC-MS测定无花果中脂肪酸组成[J].食品研究与开发, 2009, 30(3): 119-121. [Li JY. Determination of fatty acid composition in fig by GC-MS[J]. Food Research and Development, 2009, 30(3): 119-121.] DOI: 10.3969/j.issn.1005- 6521.2009.03.036.
35.王萍, 李述刚, 陆健康, 等. 南疆阿图什市两种干制无花果品质分析[J]. 农产品加工, 2015, (19): 44-46. [Wang P, Li SG, Lu JK, et al. Analysis of two kinds of dried figs quality in Atushi of Southern Xinjiang[J]. Farm Products Processing, 2015, (19): 44-46.] DOI: 10.16693/j.cnki.1671-9646(x).2015.10.016.
36.冉乾鸿, 姜波, 谭凯升, 等. 抗癌性中药材蛇莓、无花果、蒲公英脂肪酸组成研究[J]. 现代园艺, 2020, 43(21): 9-11. [Ran QH, Jiang B, Tan KS, et al. Study on fatty acid composition of anticancer chinese medicinal materials: Duchesnea indica, Ficus carica, and Taraxacum officinale[J]. Modern Horticulture, 2020, 43(21): 9-11.] DOI: 10.14051/j.cnki.xdyy.2020.21.002.
37.莫少红. 无花果研究进展[J]. 基层中药杂志, 1998, (2): 54-56. [Mo SH.Research progress on fig[J]. Primary Journal of Chinese Materia Medica, 1998, (2): 54-56.] DOI: 10.13728/j.1673-6427.1998.02.043.
38.孟正木, 王佾先, 纪江, 等. 无花果叶化学成分研究[J]. 中国药科大学学报, 1996, 27(4): 13-15. [Meng ZM, Wang YX, Ji J, et al. Studies of Chemical Constituents of Ficus Carica L[J]. Journal of China Pharmaceutical University, 1996, 27(4): 13-15.] DOI: 10.3321/j.issn:1000-5048.1996.04.003.
39.尹卫平, 冯书晓, 王水永, 等. 无花果果实中新喹啉和新酸酐化合物[J]. 中草药, 2019, 50(2): 318-323. [Yin WP, Feng SX, Wang SY, et al. New quinoline and anhydride compounds from fruits of Ficus carica[J]. Chinese Traditional and Herbal Drugs, 2019, 50(2): 318-323.] DOI: 10.7501/j.issn.0253-2670.2019.02.008.
40.戴伟娟, 司端运, 王绍红, 等. 无花果多糖对免疫抑制小鼠的免疫调节作用[J]. 中国中医药信息杂志, 2002, 9(3): 23-24. [Dai WJ, Si DY, Wang SH, et al. Immunoregulatory effects of Ficus carica polysaccharide on immunosupressive mice[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2002, 9(3): 23-24.] DOI: 10.3969/j.issn.1005-5304.2002.03.011.
41.Ou A, Zhao X, Lu Z. Autophagy is involved in Ficus carica fruit extract-induced anti-tumor effects on pancreatic cancer[J].Biomed Pharmacother, 2022, 150: 112966. DOI: 10.1016/j.biopha.2022.112966.
42.邓佳丽, 李晓, 安玉艳, 等. 无花果叶片多糖抑制胃癌细胞增殖与促进凋亡效应[J]. 天然产物研究与开发, 2021, 33(8): 1282-1291. [Deng JL, Li X, An YY, et al. Inhibition of proliferation and promotion of apoptosis of gastric cancer cells by leaf polysaccharides of Ficus carica[J]. Natural Product Research and Development, 2021, 33(8): 1282-1291.] DOI: 10.16333/j.1001-6880.2021.8.003.
43.Fawal GE, Sobhy SE, Hafez EE. Biological activities of fig latex-loaded cellulose acetate/poly (ethylene oxide) nanofiber for potential therapeutics: anticancer and antioxidant material[J]. Int J Biol Macromol, 2024, 270: 132176. DOI: 10.1016/j.ijbiomac.2024.132176.
44.Hadriche OB, Ammar S, Contreras MM, et al. HPLC-DAD-QTOF-MS profiling of phenolics from leaf extracts of two Tunisian fig cultivars: potential as a functional food[J]. Biomed Pharmacother, 2017, 89: 185-193. DOI: 10.1016/j.biopha.2017.02.004.
45.Ghorbani A. Mechanisms of antidiabetic effects of flavonoid rutin[J]. Biomed Pharmacother, 2017, 96: 305-312. DOI: 10.1016/j.biopha.2017.10.001.
46.Ramu R, Shirahatti PS, Swamy SN, et al. Assessment of in vivo antidiabetic properties of umbelliferone and lupeol constituents of Banana (Musa sp. var. Nanjangud rasa bale) flower in hyperglycaemic rodent model[J]. Plos One, 2016, 11(3): e0151135. DOI: 10.1371/journal.pone.0151135.
47.Naowaboot J, Somparn N, Saentaweesuk S, et al. Umbelliferone improves an impaired glucose and lipid metabolism in high-fat diet/streptozotocin-induced type 2 diabetic rats[J]. Phytother Res, 2015, 29(9): 1388-1395. DOI: 10.1002/ptr.5392.
48.Liu J, Lu J, Kan J, et al. Synthesis, characterization and in vitro anti-diabetic activity of catechin grafted inulin[J]. Int J Biol Macromol, 2014, 64: 76-83. DOI: 10.1016/j.ijbiomac.2013.11.028.
49.Sharma D, Tekade RK, Kalia K. Kaempferol in ameliorating diabetes-induced fibrosis and renal damage: an in vitro and in vivo study in diabetic nephropathy mice model[J]. Phytomedicine, 2020, 76: 153235. DOI: 10.1016/j.phymed.2020.153235.
50.Zang Y, Igarashi K, Li Y. Anti-diabetic effects of luteolin and luteolin-7-O- glucoside on KK-A(y) mice[J]. Biosci Biotechnol Biochem, 2016, 80(8): 1580-1586. DOI: 10.1080/09168451.2015.1116928.
51.Oza M J, Kulkarni YA. Formononetin treatment in type 2 diabetic rats reduces insulin resistance and hyperglycemia[J]. Front Pharmacol, 2018, 9: 739. DOI: 10.3389/fphar.2018.00739.
52.Qiu G, Tian W, Huan M, et al. Formononetin exhibits anti-hyperglycemic activity in alloxan-induced type 1 diabetic mice[J].Exp Biol Med, 2017, 242(2): 223-230. DOI: 10.1177/1535370216657445.
53.Babu S, Krishnan M, Rajagopal P, et al. Beta-sitosterol attenuates insulin resistance in adipose tissue via IRS-1/Akt mediated insulin signaling in high fat diet and sucrose induced type-2 diabetic rats[J]. Eur J Pharmacol, 2020, 873: 173004. DOI: 10.1016/j.ejphar.2020.173004.
54.Ramu R, Shirahatti PS, Nayakavadi S, et al. The effect of a plant extract enriched in stigmasterol and beta-sitosterol on glycaemic status and glucose metabolism in alloxan-induced diabetic rats[J]. Food Funct, 2016, 7(9): 3999-4011. DOI: 10.1039/c6fo00343e.
55.Kelany ME, Abdallah MA. Protective effects of combined beta-caryophyllene and silymarin against ketoprofen-induced hepatotoxicity in rats[J]. Can J Physiol Pharmacol, 2016, 94(7): 739-744. DOI: 10.1139/cjpp-2015-0607.
56.Zou Q, Zhang X, Liu X, et al. Ficus carica polysaccharide attenuates DSS-induced ulcerative colitis in C57BL/6 mice[J]. Food Funct, 2020, 11(7): 6666-6679. DOI: 10.1039/d0fo01162b.
57.Wang J, Wang L, Zhou J, et al. The protective effect of formononetin on cognitive impairment in streptozotocin (STZ)-induced diabetic mice[J]. Biomed Pharmacother, 2018, 106: 1250-1257. DOI: 10.1016/j.biopha.2018.07.063.
58.Yang X,Guo JL,Ye JY, et al. The effects of Ficus carica polysaccharide on immune response and expression of some immune-related genes in grass carp, Ctenopharyngodon idella [J]. Fish Shellfish Immunol, 2015, 42(1): 132-137. DOI: 10.1016/j.fsi.2014.10.037.
59.Palmeira L, Pereira C, Dias M I, et al. Nutritional, chemical and bioactive profiles of different parts of a Portuguese common fig (Ficus carica L.) variety[J]. Food Res Int, 2019, 126: 108572. DOI: 10.1016/j.foodres.2019.108572.
60.刘荣, 董崇龙. 无花果内源菌研究进展[J]. 食品安全导刊, 2019, (3): 131-132. [Liu R, Dong CL. Research progress on endogenous microorganisms in fig[J]. China Food Safety Magazine, 2019, (3): 131-132.] DOI: 10.16043/j.cnki.cfs.2019.03.117.
61.Tian R, Yang W, Xue Q, et al. Rutin ameliorates diabetic neuropathy by lowering plasma glucose and decreasing oxidative stress via Nrf2 signaling pathway in rats[J]. Eur J Pharmacol, 2016, 771: 84-92. DOI: 10.1016/j.ejphar.2015.12.021.
62.Ola MS, Ahmed MM, Ahmad R, et al. Neuroprotective effects of rutin in streptozotocin-induced diabetic rat retina[J]. J Mol Neurosci, 2015, 56(2): 440-448. DOI: 10.1007/s12031-015-0561-2.
63.Canavese M, Altruda F, Ruzicka T, et al. Vascular endothelial growth factor (VEGF) in the pathogenesis of psoriasis-a possible target for novel therapies[J]. J Dermatol Sci, 2010, 58(3): 171-176. DOI: 10.1016/j.jdermsci.2010.03.023.
64.Malecic N, Young HS. Novel investigational vascular endothelial growth factor (VEGF) receptor antagonists for psoriasis[J]. Expert Opin Investig Drugs, 2016, 25(4): 455-462. DOI: 10.1517/13543784.2016.1153064.
65.杜文静, 罗莉. 无花果叶的药用研究进展[J]. 中医药导报, 2014, 20(2): 93-95. [Du WJ, Luo L. Research progress on medicinal application of fig leave[J]. Guiding Journal of Traditional Chinese Medicine and Pharmacy, 2014, 20(2): 93-95.] DOI: 10.13862/j.cnki.cn43-1446/r.2014.02.039.
66.张蓓蓓. 植物黄酮类化合物的研究[J]. 科技视界, 2018, (23): 155-157. [Zhang BB. Study on plant flavonoids[J]. Science and Technology Vision, 2018, (23): 155-157.] DOI: 10.19694/j.cnki.issn2095-2457.2018.23.069.
67.叶华, 谢绍诗, 张文清. 无花果叶、根的药用研究进展[J]. 海峡药学, 2006, 18(6): 3-7. [Ye H, Xie SS, Zhang WQ. Medical research progress on Ficus carica leaves[J]. Strait Pharmaceutical Journal, 2006, 18(6): 3-7.] DOI: 10.3969/j.issn.1006-3765.2006.06.002.
68.Ghambarali Z, Bidmeshkipouri A, Akrami H, et al. Ethanolic extract of Ficus carica leave suppresses angiogenesis by regulating VEGF-A and integrin β3 mRNA expression in human umbilical vein endothelial cells[J]. Indian J Physiol Pharmacol, 2014, 58(4): 407-415. https://pubmed.ncbi.nlm.nih.gov/26215010/.
69.Xu XH, Zhao C, Peng Q, et al. Kaempferol inhibited VEGF and PGF expression and in vitro angiogenesis of HRECs under diabetic-like environment[J]. Braz J Med Biol Res, 2017, 50(3): e5396. DOI: 10.1590/1414-431X20165396.
70.Kumar B, Gupta SK, Srinivasan BP, et al. Hesperetin ameliorates hyperglycemia induced retinal vasculopathy via anti-angiogenic effects in experimental diabetic rats[J]. Vascul Pharmacol, 2012, 57: 201-207. DOI: 10.1016/j.vph.2012.02.007.
71.黄金勇, 吐尔逊江·达地汗, 郑靖杰, 等. 鹰嘴豆芽异黄酮对小鼠成骨前体细胞MC3T3-E1增殖及分化的研究[J]. 中国骨质疏松杂志, 2022, 28(12): 1723-1727, 1734. [Huang JY, Tuerxunjiang Dadihan, Zheng JJ, et al. Effects of isoflavone s e xtracted from chickpe a sprouts on proliferation and differentiation of mouse oste ogenic precursor cells MC3T3-E1[J]. Chinese Journal of Osteoporosis, 2022, 28(12): 1723-1727, 1734.] DOI: 10.3969/j.issn.1006-7108.2022.12.003.
72.Makoolati Z, Bahrami H, Zamanzadeh Z, et al. Efficacy of Ficus carica leaf extract on morphological and molecular behavior of mice germ stem cells[J]. Anim Reprod, 2022, 19(2): e20220036. DOI: 10.1590/1984-3143-AR2022-0036.eCollection 2022.