Preliminary study on the chemical constituents and anti-gastric cancer activity of the underground parts of Ferula songarica Pall. ex Schult.

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Author: CHANG Xiaotong ^1, ² ZHAO Yaqin ² QING Degang ² SHI Leiling ² PENG Dongge ³  WANG Guoping ²  LU Ning ⁴

Affiliation: 1.College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China 2.Xinjiang Institute of Materia Medica, Xinjiang Key Laboratory of Chinese Materia Medica and Ethnic Materia Medica, Xinjiang Key Laboratory of Traditional Chinese Medicine Resources Research and Development, Urumqi 830011, China 3.Graduate School, Xinjiang Medical University, Urumqi 830017, China 4.Department of Oncology Xinjiang, the General Hospital of Xinjiang Military Command, People's Liberation Army of China, Urumqi 830099, China

Keywords: Ferula songarica Pall. ex Schult. Chemical composition Spectral identification HGC-27 cells Anti-proliferative activity

DOI: 10.12173/j.issn.2097-4922.202601037

Reference: CHANG Xiaotong, ZHAO Yaqin, QING Degang, SHI Leiling, PENG Dongge, WANG Guoping, LU Ning.Preliminary study on the chemical constituents and anti-gastric cancer activity of the underground parts of Ferula songarica Pall. ex Schult.[J]. Yaoxue QianYan Zazhi, 2026, 30(5): 942 - 950.DOI: 10.12173/j.issn.2097-4922.202601037[Article in Chinese]  Copied

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Abstract

Objective To investigate the chemical composition of the underground parts of Ferula songarica Pall. ex Schult., and to evaluate the antiproliferative activity of its isolated compounds on gastric cancer HGC-27 cells.

Methods The air-dried, powdered underground parts of Ferula songarica Pall. ex Schult. were extracted with 95% ethanol, followed by sequential liquid-liquid partition with petroleum ether, dichloromethane, and ethyl acetate. The dichloromethane fraction was systematically isolated and purified by silica gel column chromatography combined with semi-preparative HPLC. The structures of the obtained compounds were elucidated by NMR, MS, and other spectroscopic analyses, together with their physicochemical properties. The in vitro anti-proliferative activity of the obtained compounds against human gastric cancer HGC-27 cells was evaluated using the MTT assay.

Results 5 monomeric compounds were isolated and identified, namely (5R,6R,9S,10S,11S)-1,1,5,6-tetramethyldecahydro-6,10-methanoazulen-11-yl-4-hydroxybenzoate (1), jaeschkeanadiol (2), 6-angeloyl-10-hydroxy-jaeschkeanadiol (3) lancerdiol vanillate (4), and 10-epi-γ-eudesmol (5). The evaluation results of anti-gastric cancer activity showed that compounds 1, 3, and 4 had significant inhibitory effects on the proliferation of gastric cancer HGC-27 cells, with half-maximal inhibitory concentrations (IC₅₀) of 18.55, 32.82, and 19.17 mmol/L, respectively; compound 5 exhibited only weak inhibitory activity (IC₅₀>50 mmol/L), while compound 2 showed no significant inhibitory activity (IC₅₀>100 mmol/L).

Conclusion Compound 1 is a new compound, and compounds 2 to 5 were isolated for the first time from Ferula songarica Pall. ex Schult.. In vitro antiproliferative activity evaluation shows that compounds 1, 3, and 4 have significant inhibitory effects on human gastric cancer HGC-27 cells. This study provides experimental evidence for the in-depth exploration of anti-gastric cancer active ingredients in Ferula songarica Pall. ex Schult. and the development of medicinal substitute resources for Ferula.

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