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Home Articles Vol 30,2026 No.10 Detail

Preliminary exploration of bioactive components from Cistanches Herba promoting neural stem cell proliferation

Published on Oct. 31, 2026Total Views: 41 times Total Downloads: 11 times Download Mobile

Author: HU Jiangxue 1 LIU Aoxue 1 MI Zuzheng 1 HAI Mengxue 1 LI Qiang 1

Affiliation: 1.School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China

Keywords: Cistanches Herba Neural stem cells Active ingredients Echinacoside Cistanoside A Cistanoside C

DOI: 10.12173/j.issn.2097-4922.202605065

Reference: HU Jiangxue,LIU Aoxue,MI Zuzheng,HAI Mengxue,LI Qiang. Preliminary exploration of bioactive components from Cistanches Herba promoting neural stem cell proliferation[J]. Frontiers in Pharmaceutical Sciences,2026,30(10):1644-1652. DOI:10.12173/j.issn.2097-4922.202605065[Article in Chinese]

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Abstract

Objective To investigate the active constituents of Cistanches Herba that promote the proliferation of neural stem cells (NSCs) in vitro and to preliminarily clarify the pharmacodynamic material basis.

Methods The extract of Cistanches Herba was separated into fractions with different polarities by systematic solvent extraction. NSCs were cultured in vitro, and optical density (OD) values were detected by the CCK-8 assay to calculate the proliferation rate. Using the promotion of NSC proliferation as an indicator, the active fractions were screened by an activity-guided method. The active fractions were further separated by conventional column chromatography and preparative HPLC, and the compound structures were identified by nuclear magnetic resonance (NMR) spectroscopy comparison with reference compounds. Finally, the effects of the individual compounds on NSC proliferation were evaluated by the CCK-8 assay.

Results Three active components, echinacoside, cistanoside A, and cistanoside C, were obtained. Their proliferation-promoting rates on NSCs were 48.58%, 37.06%, and 19.98%, respectively.

Conclusion The three phenylethanoid glycosides isolated from Cistanches Herba exhibit significant promoting effects on neural stem cell proliferation, providing new insight for the development of neuroprotective agents.

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References

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