Determination of three characteristic components of Ophiopogon japonicus and its adulterants in Shengmaiyin by UPLC-MS/MS

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Author: HUANG Saiyan XU Danyang YAO Yiran WANG Shu QIAN Jie ZHU Lin  LIU Huanhuan

Affiliation: Nantong Food and Drug Control Center, Nantong 226014, Jiangsu Province, China

Keywords: Shengmaiyin Ophiopogon japonicus Methylophiopogonanone A Liriopeside B Liriope muscari baily saponin C Ultra performance liquid chromatography tandem mass spectrometry

DOI: 10.12173/j.issn.2097-4922.202403050

Reference: HUANG Saiyan, XU Danyang, YAO Yiran, WANG Shu, QIAN Jie, ZHU Lin, LIU Huanhuan.Determination of three characteristic components of Ophiopogon japonicus and its adulterants in Shengmaiyin by UPLC-MS/MS[J].Yaoxue QianYan Zazhi,2024, 28(3):390-396.DOI: 10.12173/j.issn.2097-4922.202406102.[Article in Chinese]  Copied

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Abstract

Objective To evaluate the quality and authenticity of Ophiopogon japonicus in Shengmaiyin, establish ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for the determination of three characteristic components (methylophiopogonanone A, liriopeside B and liriope muscari baily saponin C), and provide technical support for drug supervision.

Methods Samples were analyzed on a Phenomenex Kinetex F5 C18 column (100 mm×3.0 mm, 2.6 µm) in a gradient elution mode with 0.1% formic acid water and -0.1% formic acid acetonitrile as the mobile phase at a flow rate of 0.4 mL/min, the column temperature was 30 ℃, and the injection volume was 1 μL. Mass spectrometer detector, electro spray ion source of positive and negative ions, and multi-reaction monitoring mode were used.

Results The linear ranges of methylophiopogonanone A, liriopeside B and liriope muscari baily saponin C were 0.016 7-1.666 0, 0.039 7-15.872 0 and 0.022 5~8.988 0 ng (r≥0.999 9), respectively. The average recovery rates of these three components were 85.16%, 86.95% and 95.07%, respectively, with RSDs of 2.65%, 1.45% and 1.14% (n=6). The content of methylophiopogonanone A in thirty-eight batches was quite different. Seven batches of liriopeside B or liriope muscari baily saponin C were detected.

Conclusion The method is simple and sensitive, and suitable for determining of three characteristic components in Shengmaiyin, which provides references for the quality control of Ophiopogon japonicus in Shengmaiyin.

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