The clinical value of UPLC-MS/MS method for the determination of plasma and tissue isoniazid concentration in lymph node tuberculosis patients

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Author: Yu ZHANG ¹ Xiao-Yu LIU ² Feng XU ² Huan ZHANG ¹ Qiao-Yan DING ¹ Li-Hua MA ¹ Si-Si LI ¹  Ming ZHOU ¹

Affiliation: 1. Department of Pharmacy, Wuhan Pulmonary Hospital, Wuhan 430030, China 2. Department of Surgical, Wuhan Pulmonary Hospital, Wuhan 430030, China

Keywords: Lymph node tuberculosis Isoniazid Ultra performance liquid chromatography-mass spectrum/mass spectrum

DOI: 10.12173/j.issn.1008-049X.202303215

Reference: Yu ZHANG, Xiao-Yu LIU, Feng XU, Huan ZHANG, Qiao-Yan DING, Li-Hua MA, Si-Si LI, Ming ZHOU.The clinical value of UPLC-MS/MS method for the determination of plasma and tissue isoniazid concentration in lymph node tuberculosis patients[J].Zhongguo Yaoshi Zazhi,2023, 26(11):311-318.DOI: 10.12173/j.issn.1008-049X.202303215.[Article in Chinese]  Copied

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Abstract

Objective To investigate the correlation between isoniazid concentration in plasma and lymph node tissue of patients with lymph node tuberculosis, and to explore its clinical value.

Methods The basic information of patients with lymph node tuberculosis in our hospital and venous blood samples and neck lymph node tissue samples at different time points were collected. UPLC-MS/MS method was established and isoniazid concentration in plasma and neck lymph node tissue samples was quantitatively detected, and the correlation between isoniazid concentration in plasma and lymph node tissue was analyzed.

Results The linear range of isoniazid blood concentration and lymph node tissue concentration were 0.25-16 μg·mL^-1 (r=0.999 8) and 2-128 μg·g^-1 (r=0.998 8), respectively. The precision, accuracy, and matrix effect of each quality control sample met the requirements. Plasma isoniazid concentration and lymph node tissue isoniazid concentration were significantly correlated at 10 min (r_s= 0.501 1, P=0.001), 30 min (r_s= 0.402 8, P=0.005) and 60 min (r_s= 0.614 6, P=0.001) after intravenous infusion of isoniazid. The ratio of lymph node tissue isoniazid concentration to plasma isoniazid concentration was 1.46 (0.62, 3.55) mL·g^-1 at 10 min. At 30 min, the ratio was 5.25 (4.61, 11.61) mL·g^-1. At 60 min, the ratio was 6.62 (4.42, 10.78) mL·g^-1.

Conclusion The established UPLC-MS/MS method has good specificity, high sensitivity, accurac and precision. Monitoring plasma isoniazid concentration provides a reference for the rational use of isoniazid in patients with lymphatic tuberculosis.

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1.陆伟, 周扬, 刘剑君. 新中国成立70年来我国结核病防治工作的进展与成就[J]. 中华疾病控制杂志, 2019, 23(7): 754-757, 762. [Lu W, Zhou Y, Liu JJ. The progress and achievements of tuberculosis control in China since 1949[J]. Chinese Journal of Disease Control & Prevention, 2019,23(7): 754-757, 762] DOI: 10.16462/j.cnki.zhjbkz. 2019.07.003.

2.Ramírez-Lapausa M, Menéndez-Saldaña A, Noguerado-Asensio A. Tuberculosis extrapulmonar, una revisión [Extrapulmonary tuberculosis] [J]. Rev Esp Sanid Penit, 2015, 17(1): 3-11. DOI: 10.4321/S1575-06202015000100002.

3.Ganchua SKC, White AG, Klein EC, et al. Lymph nodes-the neglected battlefield in tuberculosis[J]. PLoS Pathog, 2020, 16(8): e1008632. DOI: 10.1371/journal.ppat.1008632.

4.邵琤, 薛宗锡, 游佩涛, 等. 240例颈淋巴结结核的回顾性分析[J].新疆医学, 2016, 46(9): 1170-1173. [Shao Z, Xue ZX, You PT, et al. A clinical review of 240 cases of cervical tuberculous lymphadenitis[J]. Xinjiang Medical Journal, 2016, 46(9): 1170-1173.] DOI: CNKI:SUN: XJYI.0.2016-09-027.

5.高璐珏, 黄子慧, 高敏行, 等. 浅表淋巴结结核患者401例临床资料分析与治疗方法探析[J]. 中华中医药杂志, 2020, 35(10): 5214-5219. [Gao LJ, Huang ZH, Gao MX, et al. Analysis of clinical data and exploration of treatment methods in 401 patients with superficial tuberculous lymphadenitis[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2020, 35(10): 5214-5219.] https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFD&filename=BXYY202010123.

6.Jawahar MS, Rajaram K, Sivasubramanian S, et al. Treatment of lymph node tuberculosis--a randomized clinical trial of two 6-month regimens[J]. Trop Med Int Health, 2005, 10(11): 1090-1098. DOI: 10.1111/j.1365-3156.2005.01493.x.

7.韩丹. 异烟肼用于颈淋巴结结核换药60例疗效观察[J]. 内蒙古中医药, 2011, 30(18): 102-103. [Han D. Therapeutic effect of isoniazid on 60 cases of cervical lymph node tuberculosis[J]. Inner Mongolia Journal of Traditional Chinese Medicine, 2011, 30(18): 102-103.] DOI: 10.16040/j.cnki.cn15-1101.2011.18.139.

8.Bergogne-Bérézin, E. Predicting the efficacy of antimicrobial agents in respiratory infections—is tissue concentration a valid measure[J]. J Antimicrob Chemother, 1995, 35(3): 363-371. DOI: 10.1093/jac/35.3.363.

9.唐神结, 主编. 临床结核病学. 第2版[M]. 北京:人民卫生出版社, 2019: 478-491.

10.中国药典2020年版. 四部[S]. 2020: 466-472.

11.Ketata W, Rekik WK, Ayadi H, et al. Extrapulmonary tuberculosis[J]. Rev Pneumol Clin, 2015, 71(2-3): 83-92. DOI: 10.1016/j.pneumo.2014.04.001.

12.刘丽红. 彩色多普勒超声诊断颈部淋巴结结核的临床价值[J]. 影像研究与医学应用, 2022, 6(12): 64-66. [Liu LH. Clinical value of color Doppler ultrasound in the diagnosis of cervical lymph node tuberculosis[J]. Journal of Imaging Research and Medical Applications, 2022, 6(12): 64-66.] DOI: 10.3969/j.issn.2096-3807.2022.12.021.

13.黄海荣, 王隽, 初乃惠. 结核病患者N-乙酰基转移酶2编码基因多态性检测与异烟肼合理用药专家共识[J]. 中国防痨杂志, 2021, 43(11): 1107-1112. [Huang HR, Wang J, Chu NH. Expert consensus on polymorphism detection of N-acetyltransferase-2 encoding gene and appropriate isoniazid dosing for tuberculosis patients[J]. Chinese Journal of Antituberculosis, 2021, 43(11): 1107-1112.] DOI: 10.3969/j.issn.1000-6621.2021.11.001.

14.Alshaikheid M, Romdhane HB, Fredj NB, et al. Isoniazid therapeutic drug monitoring in Tunisian patients with tuberculosis[J]. J Clin Pharmacol, 2021, 61(7): 972-978. DOI: 10.1002/jcph.1813.

15.梁英杰, 亓琳, 韩水, 等. HPLC-MS/MS法同时测定人血清中4种一线抗结核药物浓度及其临床应用 [J]. 南开大学学报(自然科学版), 2020, 53(3): 49-57. [Liang YJ, Qi L, Han S, et al. Simultaneous determination of four first-line anti-tuberculosis drugs in human serum by HPLC-MS/MS and its clinical application[J] Acta Scientiarum Naturalium Universitatis Nankaiensis (Natural Science Edition), 2020, 53(3): 49-57.] http://qikan.cqvip.com/Qikan/Article/Detail?id=7102198257.

16.Ungcharoen U, Sriplung H, Mahasirimongkol S, et al. The influence of NAT2 genotypes on isoniazid plasma concentration of pulmonary tuberculosis patients in Southern Thailand[J]. Tuberc Respir Dis (Seoul), 2020, 83(Supple 1): S55-S62. DOI: 10.4046/trd.2020.0068.

17.Hansen KK, Nielsen F, Stage TB, et al. Microdialysis as a tool to determine the local tissue concentration of dicloxacillin in man[J]. Br J Clin Pharmacol, 2017, 84(3): 533-541. DOI: 10.1111/bcp.13468.

18.龙芳芳, 王以美, 于锋, 等. 异烟肼抗结核治疗致肝损伤的国内文献综合分析[J]. 药物流行病学杂志, 2016, 25(6): 385-390. [Long FF, Wang YM, Yu F, et al. Systematic review of isoniazid induced liver injury in China[J]. Chinese Journal of Pharmacoepidemiology, 2016, 25(6): 385-390.] DOI: 10.19960/j.cnki.issn1005-0698.2016.06.014.

19.黄银, 陈诚, 杨娇, 等. 多黏菌素治疗呼吸机相关性肺炎患者肺组织浓度与血药浓度关系[J]. 医药导报, 2018, 37(10): 1256-1261. [Huang Y, Cheng C, Yang J, et al. Relationship of polymyxins between intrapulmonary concentration and blood concentration in patients with ventilator-associated pneumonia[J]. Herald of Medicine, 2018, 37(10): 1256-1261.] DOI: CNKI:SUN:YYDB.0. 2018-10-032.

20.雷倩, 赵嫄, 王皓, 等. 3种二线抗结核药物血药浓度测定及其影响因素[J]. 药物分析杂志, 2020, 40(8): 1405-1412. [Lei Q, Zhao Y, Wang H, et al. Determination of plasma concentration of three second-line anti-tuberculous drugs and their influencing factors[J]. Chinese Journal of Pharmaceutical Analysis, 2020, 40(8): 1405-1412.] DOI: 10.16155/j.0254-1793.2020.08.10.